Project description:Viromes of sour and sweet cherry trees in Hungarian germ line collections were surveyed using small RNA HTS as an unbiased method. RNA from leaf samples of different cultivars were purified and used to produce seven pools from which small RNA HTS libraries were prepared. The sequenced reads were analyzed using bioinformatic methods to revel the presence of viruses in the samples. Presence of the viruses were validated using RT-PCR.
Project description:The placenta is a dynamic reproductive organ for cattle reproduction and is indispensable for embryonic blood supply, nutrition transportation, and fetal calf development, and it directly affects the development of fetal calf in utero. However, the detailed molecular mechanism of placenta act on fetal calf still remains unclear.In the present study,we used the proteome of placenta to identify the key gene and protein in Low weight(LW) fetal calves and High weight(HW) fetal calves, and reveal the fetal calf growth-related candidate biomarkers by integrative analysis.Placenta proteomics provide new insight into the physiological mechanisms and potential biomarkers for fetal calf development in utero,which can be employed to enhance breeding efficiency and genetic improvement in cattle.
Project description:We assessed the transcriptomic adaptation of the calf rumen epithelium to changes in ruminal pH caused by feeding calf starter with and without forage during weaning transition.