Project description:The placenta is a dynamic reproductive organ for cattle reproduction and is indispensable for embryonic blood supply, nutrition transportation, and fetal calf development, and it directly affects the development of fetal calf in utero. However, the detailed molecular mechanism of placenta act on fetal calf still remains unclear.In the present study,we used the proteome of placenta to identify the key gene and protein in Low weight(LW) fetal calves and High weight(HW) fetal calves, and reveal the fetal calf growth-related candidate biomarkers by integrative analysis.Placenta proteomics provide new insight into the physiological mechanisms and potential biomarkers for fetal calf development in utero,which can be employed to enhance breeding efficiency and genetic improvement in cattle.
Project description:We assessed the transcriptomic adaptation of the calf rumen epithelium to changes in ruminal pH caused by feeding calf starter with and without forage during weaning transition.
Project description:Harnessing maternal blood to predict fetal growth is an emerging area in livestock research, offering a non-invasive approach to monitor fetal development and potentially enhance reproductive management in cattle. In this study, blood samples were collected from 20 recipient cows carrying female fetuses produced via in vitro embryo transfer at 63 days of gestation. At birth, calf weights ranged from 30 to 72.5 kg, with an average of 45 kg. RNA-seq analysis of maternal blood revealed transcriptomic patterns associated with calf birth weight. Furthermore, a transcriptomic signature was identified that enables accurate prediction of calf birth weight with low error.
Project description:Most cell culture experiments utilize media containing fetal calf serum. Results are often interpreted regarding importance to human pathways. We studied gene expression in mouse macrophages grown in the absence of serum, and in fetal calf serum, mouse serum, and human serum using genome wide expression systems in resting conditions and after stimulation with lipopolysaccharide.