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Transcriptional study of the home-use TriPollar radiofrequency device on mice skin

ABSTRACT: Transcriptional study of the home-use TriPollar radiofrequency device on mice skin

PROVIDER: PRJNA1165663 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30805556_1.fastq.gz	Fastqsanger.gz
	SRR30805556_2.fastq.gz	Fastqsanger.gz
	SRR30805557_1.fastq.gz	Fastqsanger.gz
	SRR30805557_2.fastq.gz	Fastqsanger.gz
	SRR30805558_1.fastq.gz	Fastqsanger.gz

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Transcriptional study of the home-use TriPollar radiofrequency device on mice skin

Project description:Radiofrequency technology has shown notable efficacy in addressing skin aging. Despite its effectiveness, there is a lack of transcriptional studies exploring how radiofrequency technology enhances skin aging. This self-control study utilized ICR male mice divided into two age groups (four-month-old and one-year-old) to assess the impact of Tripollar Stop VX2 radiofrequency device on skin. The treatment was conducted every other day for one week with device set on low-range mode.

2025-05-22 | GSE278079 | GEO

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Project description:The modification of skin identity, such as the conversion of residual limb/stump (non-volar) skin of amputees to pressure-resistant palmoplantar skin to enhance prosthesis use and minimize skin breakdown, has tremendous clinical potential. Recognizing the capacity of fibroblasts to modify keratinocyte differentiation, we hypothesized that volar fibroblast injections might induce ectopic volar characteristics. First, we observed pressure-responsive volar fibroblast resulting in increased limb development after pressure treatment, then we confirmed this in using a bioprinted skin construct, and later in a clinical trial that showed increased volar measures in non-volar skin after volar fibroblast injections. Bulk and single cell RNA seq demonstrate gene ontology categories of cornified envelope, keratinization and morphogenic pathways. Thus, the long-term engraftment of volar fibroblasts creates a platform for the therapeutic development

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2024-09-09 | GSE231996 | GEO

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N7-Methylguanosine tRNA modification promotes hepatocellular carcinoma metastasis after insufficient radiofrequency ablation

Project description:Radiofrequency heat ablation is an ideal radical cure for HCC treatment; however, insufficient radiofrequency ablation (IRFA) could lead to a high recurrence rate. N7-methylguanosine (m7G) on tRNAs is a heat-responding modification that is critical for yeast survival under high temperature, while its function and mechanism in HCC recurrence after IRFA are unknown. Here, we found that IRFA significantly up-regulates the level of m7G tRNA modification and its methyltransferase complex components METTL1 and WDR4 in multiple systems including HCC patient-derived xenograft (PDX) mouse, HCC tissues of patients, sublethal-heat-treated models of HCC cell lines and organoids. Functionally, gain- or loss-of function assays showed that METTL1 mediated m7G tRNA modification promotes HCC metastasis under sublethal heat exposure both in vitro and in vivo. Mechanistically, we found that METTL1 and m7G tRNA modification enhance the translation of SLUG and SNAIL in a codon frequency dependent manner under sublethal heat exposure. Overexpression of SLUG and SNAIL rescued the malignant potency of METTL1 knockdown HCC cells after sublethal heat stress. Our study uncovers the important physiological functions of m7G tRNA modification in heat stress responses and HCC recurrence after IRFA and suggests that targeting METTL1-m7G-SLUG and SNAIL axis could be a promising strategy to prevent HCC metastasis after radiofrequency heat ablation treatment.

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