Project description:The study was conducted on a model of Lactiplantibacillus plantarum, one of the most studied species widely used in the food industry as a probiotic microorganism and/or microbial starter culture. As a result of step-by-step selection from the L. plantarum 8p-a3 strain isolated from the «Lactobacterin» probiotic, the L. plantarum 8p-a3-Clr-Amx strain was obtained, showing increased resistance, compared with the parent strain, to amoxicillin-clavulanic acid (MIC 20 mcg/ml) and clarithromycin (MIC 10 mcg/ml). The L. plantarum strain DMC-S1 was isolated from the intestine of Drosophila melanogaster Canton-S line. Extracellular vesicles of this bacterium can play a significant role in the drug-resistance development and host-microbe interactions.
Project description:The study evaluates the effect of Lactiplantibacillus plantarum IMC 510® supplementation on anthropometric and biochemical parameters, GM composition and gastrointestinal and general symptoms of overweight/obese subjects.
Project description:Identification of proteins contained in extracellular vesicles of Lactiplantibacillus plantarum PCM 2675. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).
Project description:This study was conducted to analyze phenotypic and proteomic differences of two Lactiplantibacillus plantarum strains (WCFS1, model strain from human saliva, and CIP104448, stool isolate) when a biofilm was produced under static conditions (well researched), or with the addition of flow (novel).
Project description:Strains of Lactiplantibacillus plantarum treated with lithium chloride to extract non-covalently bound cell wall proteins that were identified by mass spectrometry proteomic analysis.
Project description:Cyclic di-adenosine monophosphate (c-di-AMP) is a crucial second messenger used by bacteria to mediate bacterial-host interactions. However, its roles in Lactiplantibacillus plantarum remain poorly understood. Here, we identify RwpL, which consists solely of an HTH domain, as a c-di-AMP receptor in L. plantarum WCFS1. RwpL mediates epithelial adhesion of L. plantarum WCFS1 and functions as both a transcription inhibitor and activator, with transcriptional output determined by the position of its binding site relative to the transcription start site (TSS) of target genes. Specifically, RwpL inhibits wxlA transcription by binding downstream of its TSS, thus reducing WxlA interactions with fibronectin, a matrix protein on the surface of intestinal epithelia. In contrast, RwpL activates transcription of the matrix-degrading metalloprotease gene mmpL by recruiting the sigma factor RpoA upstream of its TSS. Notably, elevated c-di-AMP levels diminish RwpL binding affinity for both DNA and RpoA, thereby reversing its regulatory functions and enhancing L. plantarum adhesion to host epithelial cells. Together, our findings uncover a dual and reversible regulatory mechanism through which c-di-AMP modulates bacterial-host interactions in L. plantarum.