Project description:Numerous studies have demonstrated that golden pompano (Trachinotus blochii) is sensititive to hypoxia, which causes a devastating blow to the golden pompano industry. And different methods of reoxygenation after hypoxia could bring differnt effects on metabolism for golden pompano.
Project description:Emerging infectious diseases pose a significant threat to both human and animal populations. Rapid de novo identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens. Here, we have developed a universal pipeline by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid de novo identification of bacterial protective proteins from a clinical isolate without prior knowledge of the pathogenic bacterium. As a proof of concept, we identified 3 novel protective proteins of A. pleuropneumoniae. Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against A. pleuropneumoniae. We believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.
Project description:Microsatellite characterisation and sex-typing in two invasive parakeet species, the monk parakeet, Myiopsitta monachus, and the ring-necked parakeet, Psittacula krameri.
