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Effect of crpPg gene deletion on Porphyromonas gingivalis gene expression

ABSTRACT: Effect of crpPg gene deletion on Porphyromonas gingivalis gene expression

PROVIDER: PRJNA1186201 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31354006_1.fastq.gz	Fastqsanger.gz
	SRR31354006_2.fastq.gz	Fastqsanger.gz
	SRR31354007_1.fastq.gz	Fastqsanger.gz
	SRR31354007_2.fastq.gz	Fastqsanger.gz
	SRR31354008_1.fastq.gz	Fastqsanger.gz

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Effect of crpPg gene deletion on Porphyromonas gingivalis gene expression

Project description:Porphyromonas gingivalis is one of the main etiological agents responsible for initiating and progressing periodontal diseases in humans. This bacterium inhabits unfavorable conditions, therefore, to adapt to the changing external environment, e.g. redox conditions, and nutrient limitations, it must respond quickly to these stress factors. For this purpose, it uses transcription regulators. One of them can be the CrpPg protein classified to the CRP/FNR protein superfamily. The goal of this study was to investigate the properties of the CrpPg protein and identify the regulatory pathways it influences related to P. gingivalis virulence. For this, we generated ΔcrpPg mutant strain (A7436ΔcrpPg ) in the A7436 wild-type. Using RNA sequencing (RNA-seq), we examined how the deletion of crpPg affects gene expression in P. gingivalis.

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Project description:Porphyromonas gingivalis is one of the main etiological agents responsible for initiating and progressing periodontal diseases in humans. P. gingivalis does not synthesize heme, and it uses host hemoproteins as a source of this molecule. The main heme uptake mechanism utilized by P. gingivalis is the Hmu system composed of six proteins where HmuY (hemophore-like protein) and HmuR (TonB-dependant outer membrane heme receptor) play a crucial role in heme supply. The second heme uptake mechanism is the Hus system composed of four proteins, with HusA (hemophore-like protein) and HusB (TonB-dependant outer membrane receptor) supporting heme uptake. Heme and iron starvation influence the P. gingivalis phenotype, therefore the aim was to determine the influence of deletion of HmuY and HmuR (ΔhmuYR strain) or HusA and HusB (ΔhusAB strain) encoding genes on P. gingivalis gene expression.

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Analysis of gene expression in Porphyromonas gingivalis hemH deletion mutant strain grown under heme-depleted conditions (RNA-seq analysis)

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