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Development of stem cell derived type alveolar epithelial cells (AEC2s) for pulmonary fibrosis modeling

ABSTRACT: Development of stem cell derived type alveolar epithelial cells (AEC2s) for pulmonary fibrosis modeling

PROVIDER: PRJNA1200147 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31770128_1.fastq.gz	Fastqsanger.gz
	SRR31770128_2.fastq.gz	Fastqsanger.gz
	SRR31770129_1.fastq.gz	Fastqsanger.gz
	SRR31770129_2.fastq.gz	Fastqsanger.gz
	SRR31770130_1.fastq.gz	Fastqsanger.gz

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Project description:Idiopathic pulmonary fibrosis (IPF) is an irreversible lung condition that progresses over time, which ultimately results in respiratory failure and mortality. In this study, we found that PLAC8 was downregulated in the lungs of IPF patients based on GEO data, in bleomycin (BLM)-induced lungs of mice, and in primary murine alveolar epithelial type II (pmATII) cells and human lung epithelial cell A549 cells. Overexpression of PLAC8 facilitated autophagy and inhibited apoptosis of pmATII cells and A549 cells in vitro. Moreover, inhibition of autophagy or overexpression of p53 partially abolished the effects of PLAC8 on cell apoptosis. ATII cell-specific overexpression of PLAC8 alleviated BLM-induced pulmonary fibrosis in mice. Mechanistically, PLAC8 interacts with VCP-UFD1-NPLOC4 complex to promote p53 degradation and facilitate autophagy, resulting in inhibiting apoptosis of alveolar epithelial cells and attenuating pulmonary fibrosis. In summary, these findings indicate that PLAC8 may be a key target for therapeutic interventions in pulmonary fibrosis.

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Project description:AEC2s are putatively major cells in development of lung diseases like Idiopathic Pulmonary Disease (IPF). For studying IPF-related phenotypes of AEC2s, We used differentiated AEC2s (diff-AEC2s) with some merits compared to conventional in vitro alveolar cells and bleomycin as a pulmonary fibrosis-inducing agent. With bleomycin treatment for 24 hr, some fibrotic phenotypes, senescent and transtional markers like Ctgf, Lgals3, and Krt8 were elevated with dose-dependent manner in RNA and protein expression. Also, up-regulation of P53 pathway and some irregular cell cycle expreesion were identified with bleomycin treatment.

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