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Immunostimulatory effects of Streptococcus sanguinis extracellular membrane vesicles protect oral gingival epithelial cells from periodontal pathobiont damage

ABSTRACT: Immunostimulatory effects of Streptococcus sanguinis extracellular membrane vesicles protect oral gingival epithelial cells from periodontal pathobiont damage

PROVIDER: PRJNA1217034 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32173754_1.fastq.gz	Fastqsanger.gz
	SRR32173754_2.fastq.gz	Fastqsanger.gz
	SRR32173755_1.fastq.gz	Fastqsanger.gz
	SRR32173755_2.fastq.gz	Fastqsanger.gz
	SRR32173756_1.fastq.gz	Fastqsanger.gz

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Immunostimulatory effects of Streptococcus sanguinis extracellular membrane vesicles protect oral gingival epithelial cells from periodontal pathobiont damage

Project description:Gingival epithelial cells internalize Streptococcus sanguinis EMVs within 24 hours of inoculation, resulting in an immune response that does not cause cell death. To look at the global transcriptomic response to S. sanguinis EMVs, we performed an RNA sequencing experiment comparing SK36 EMV and PBS-inoculated TIGK cells.

2025-02-12 | GSE288367 | GEO

Immunostimulatory effects of <i>Streptococcus sanguinis</i> extracellular membrane vesicles protect oral gingival epithelial cells from periodontal pathobiont damage.

Project description:The commensal Streptococcus sanguinis is highly prevalent in the oral cavity and characterized for its ability to inhibit growth of oral pathogens. Like many other cell types, streptococci produce extracellular membrane vesicles (EMVs), which contain specific molecular cargo and facilitate interactions with host cells. We previously demonstrated that EMVs from S. sanguinis are internalized by gingival epithelial cells (GECs) without causing cell death. Our aim is to characterize the effects of vesicles on eukaryotic cells. Microscopy studies of gingival epithelial cells inoculated with EMVs from wild-type and specific deletion mutants show differential uptake, with decreased uptake of ΔSSA1099 EMVs and increased uptake of ΔSSA1882 EMVs relative to SK36 EMVs. However, EMVs from wild-type and deletion mutants showed similar patterns in cytokine and chemokine secretion. Transcriptomic analysis of gingival epithelial cells inoculated with SK36 EMVs showed a downregulation of genes implicated in apoptosis as well as interferon signaling, while showing an upregulation of genes involved in cytokine production. Gelatin zymography results show that SK36 EMVs have a contrasting result on production of MMP2/9; MMP2 production is decreased while MMP9 is increased by 48 hours post-inoculation (hpi). Dual-inoculation studies demonstrate that prior internalization of S. sanguinis EMVs protects gingival epithelial cells from exposure to pathobiont Porphyromonas gingivalis outer membrane vesicles (OMVs), preventing dissociation and cell death. Our overall findings suggest that S. sanguinis EMVs trigger an immune response on gingival epithelial cells; however, this response suggests inhibition of some immune signaling pathways. Our results highlight an important role in commensalism, in which a microbe induces an immune response but avoids damage to host cells, thus discouraging infection by pathobionts.

| S-EPMC11895462 | biostudies-literature

Proteomic characterization of extracellular membrane vesicles from Streptococcus sanguinis

Project description:Streptococcus sanguinis, a Gram-positive oral commensal, is highly prevalent in the oral cavity and well-characterized for its ability to antagonize oral pathobionts. We have found that production and dissemination of membrane vesicles by S. sanguinis is dependent on environmental and community factors. Co-culture with interacting commensal Corynnyebacterium durum, as well as with the periodontal pathobiont Filifactor alocis had no effect on S. sanguinis vesicle number and size, whereas the periodontal pathobiont Porphyromonas gingivalis abolished S. sanguinis vesicle production. Using both correlation and differential expression analyses to examine the transcriptomic changes underlying vesicle production, we found that differential expression of genes encoding proteins related to the cytoplasmic membrane and peptidoglycan correlate with the abundance of membrane vesicles. Proteomic characterizations of the vesicle cargo identified a variety of proteins, including those predicted to influence host interactions or host immune responses. Cell culture studies of gingival epithelial cells demonstrated that both crude and highly purified membrane vesicles could induce the expression of IL-8, TNF-α, IL-1β, and Gro-α within 6 hours of inoculation at levels comparable to whole cells. OTaken together, our findings suggest that production of membrane vesicles by S. sanguinis is heavily influenced by community and environmental factors, and plays an important role in communication with host cells.

2024-10-17 | PXD041791 | Pride

Differential DNA methylation in gingival tissues in periodontal health and disease [Array]

Project description:We investigated differential DNA methylation in gingival tissues in states of periodontal health, gingivitis and periodontitis.

2021-04-23 | GSE173081 | GEO

Differential mRNA transcription in gingival tissues in periodontal health and disease [RNA-seq]

Project description:We investigated differential mRNA expression in gingival tissues in states of periodontal health, gingivitis and periodontitis.

2021-04-23 | GSE173078 | GEO

ssa_1972-null mutant of Streptococcus sanguinis SK36

2013-07-17 | GSE48940 | GEO

ssa_1972-null mutant of Streptococcus sanguinis SK36

Project description:Transcriptional profiling of ssa_1972-null mutant of Streptococcus sanguinis compared with wild type. The ssa_1972 gene was inactivated in Streptococcus sanguinis SK36 and transcriptional profile was compared with wild type SK36. More information can be found at http://www.people.vcu.edu/~pingxu One-condition experiment, M-NM-^Tssa_1972 vs S. sanguinis SK36 cells. Biological replicates: 3 wild type, 3 M-NM-^Tssa_1972, independently grown and harvested. One replicate (one wild type and one M-NM-^Tssa_1972 mixture) per array.

2013-07-17 | E-GEOD-48940 | biostudies-arrayexpress

Identification of Differentially Expressed Genes in Human Gingival and Periodontal Derived Stromal Cells Compared with Human Dermal Fibroblasts

Project description:The normal epithelium derived stromal cells included ECM, normal fibroblast, mesenchymal stromal cells and osteoblast also plays a significant role in the progress of cancers but poorly investigated in the progress of OSCC. In this study, the stromal cells derived from gingival and periodontal tissues were extracted and human dermal fibroblasts were selected as control group. The differentially expressed genes in Gingival derived stromal cells and Periodontal derived stromal cells were identified by microarray. Furthermore, the biological process, cellular component, molecular function and cell pathways of differentially expressed genes in gingival derived stromal cells and periodontal derived stromal cells were analyzed by GO enrichment analysis and KEGG enrichment analysis respectively. All of these findings could provide potential genes in gingival derived stromal cells and periodontal derived stromal cells that influences the progress of OSCC.

2022-08-23 | GSE174595 | GEO

Physiological and molecular characterization of genetic competence in Streptococcus sanguinis

Project description:Streptococcus sanguinis is a major component of the oral flora and an important cause of infective endocarditis. The genome sequence of S. sanguinis strain SK36 was recently determined. A number of foreign genes acquired by natural transformation were detected, as well as orthologs of competence genes previously identified in other species. However, significant differences in the S. sanguinis competence system relative to that of other streptococci were noted. We sought to examine S. sanguinis genetic competence, to characterize the global transcriptional response to competence induction, and to compare our results with those obtained from previous analyses of other streptococci. A mutant possessing an in-frame deletion in the comC gene encoding the competence-stimulating peptide was created and confirmed to have the expected phenotype. Studies indicated that competence could be induced in this strain by addition of competence-stimulating peptide, and determined the optimal conditions to employ for this purpose. Expression was monitored by microarray analysis at multiple time points from 2.5 to 30 min after induction. Over 200 genes were identified whose expression was altered at least two-fold in at least one time point, with the majority upregulated. The M-bM-^@M-^\lateM-bM-^@M-^] response was typical of that seen in previous studies. However, comparison of the M-bM-^@M-^\earlyM-bM-^@M-^] response in S. sanguinis with that of other streptococci revealed unexpected heterogeneity with regard to the number of genes induced, the nature of these genes, and their putative upstream regulatory sequences. S. sanguinis possesses a comparatively limited early response, which may define a minimal competence regulatory circuit. Transcriptional analysis of S. sanguinis strain JFP41 cells 0 to 30 min after treatment with CSP. Biological replicates: 3 replicates each independently grown and harvested. 4 technical replicates per array

2011-01-21 | E-GEOD-21619 | biostudies-arrayexpress

Physiological and molecular characterization of genetic competence in Streptococcus sanguinis

Project description:Streptococcus sanguinis is a major component of the oral flora and an important cause of infective endocarditis. The genome sequence of S. sanguinis strain SK36 was recently determined. A number of foreign genes acquired by natural transformation were detected, as well as orthologs of competence genes previously identified in other species. However, significant differences in the S. sanguinis competence system relative to that of other streptococci were noted. We sought to examine S. sanguinis genetic competence, to characterize the global transcriptional response to competence induction, and to compare our results with those obtained from previous analyses of other streptococci. A mutant possessing an in-frame deletion in the comC gene encoding the competence-stimulating peptide was created and confirmed to have the expected phenotype. Studies indicated that competence could be induced in this strain by addition of competence-stimulating peptide, and determined the optimal conditions to employ for this purpose. Expression was monitored by microarray analysis at multiple time points from 2.5 to 30 min after induction. Over 200 genes were identified whose expression was altered at least two-fold in at least one time point, with the majority upregulated. The “late” response was typical of that seen in previous studies. However, comparison of the “early” response in S. sanguinis with that of other streptococci revealed unexpected heterogeneity with regard to the number of genes induced, the nature of these genes, and their putative upstream regulatory sequences. S. sanguinis possesses a comparatively limited early response, which may define a minimal competence regulatory circuit.

2011-01-21 | GSE21619 | GEO

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