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Genetic Analysis of Variation in Transcription Factor Binding in Yeast

ABSTRACT: Genetic Analysis of Variation in Transcription Factor Binding in Yeast

PROVIDER: PRJNA122523 | ENA |

REPOSITORIES: ENA

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Genetic variation at transcription factor binding sites largely explains phenotypic heritability in maize

Project description:Comprehensive maps of functional variation at transcription factor (TF) binding sites (cis-elements) are crucial for elucidating how genotype shapes phenotype. Here, we report the construction of a pan-cistrome of the maize leaf under well-watered and drought conditions. We quantified haplotype-specific TF footprints across a pan-genome of 25 maize hybrids and mapped over two hundred thousand (epi)genetic variants (termed binding-QTL) linked to cis-element occupancy. Three lines of evidence support the functional significance of binding-QTL: i) coincidence with causative loci that regulate traits, including VGT1, ZmTRE1, and the MITE transposon near ZmNAC111 under drought; ii) bQTL allelic bias is shared between inbred parents and matches ChIP-seq results, iii) partitioning genetic variation across genomic regions demonstrates that binding-QTL capture the majority of heritable trait variation across ~72% of 143 phenotypes. Our study provides an auspicious approach to make functional cis-variation accessible at scale for genetic studies and targeted engineering of complex traits.

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Genetic Analysis of Variation in Transcription Factor Binding in Yeast

Project description:This SuperSeries is composed of the SubSeries listed below.

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