ABSTRACT: The tolerance mechanism of diarrhetic shellfish toxins mediated by the extracellular regulated protein kinase (ERK) pathway in the mussel Perna viridis
Project description:Here, we integrated high-throughput transcriptome and proteome sequencing to construct a comprehensive protein database for the byssus of Chinese green mussel (Perna viridis), aiming at providing novel insights into the molecular mechanisms of byssal binding to heavy metals.
Project description:This project aimed to disclose the metabolic alterations and responses of the marine mussel Mytilus galloprovincialis after grazing the toxic microalga Prorocentrum lima. Mussels metabolic alterations were investigated by shotgun proteomics, during the phases of intoxication and depuration. The diarrhetic shelllfisf toxins were also quantified in this project, for assessing the levels of contamination of mussels.
2020-12-17 | PXD022293 | Pride
Project description:Transcriptomic profile of the cockle Cerastoderma edule exposed to Diarrhetic Shellfish Toxins seasonal contamination
| PRJNA739261 | ENA
Project description:de novo transcriptome assembly of green mussel, Perna viridis
Project description:Mollusk secretes a periostracum layer prior to the underlying calcified shell. This organic membrane serves as the first line of protection and primary template for shell orchestration. However, the chemical composition and formation mechanism of the periostracum layer is largely unknown. In this study, we applied transcriptomic, proteomics, physical and chemical analysis to unravel the mysteries of the periostracum formation in the green mussel Perna viridis (Linnaeus). Scanning electron microscopy examination and FTIR analysis showed that the periostracum layer was a multilayered organic membrane composed of polysaccharides, lipids and proteins. Interestingly, proteomic study identified components enriched in tyrosine and some enzymes evolved in tyrosine oxidation, indicating that tyrosine oxidation might play an important role in the periostracum formation. Moreover, comparative transcriptomics suggested that tyrosine-rich proteins were intensively synthesize in the periostracum groove. After being secreted, the periostracum proteins were gradually tanned by oxidation in the sea water, and the level of crosslink increased significantly as revealed by the ATR-FTIR. Our present study sheds light on the chemical composition and putative tanning mechanism of the periostracum layer in bivalve mollusk.
2022-10-20 | GSE215890 | GEO
Project description:Whole genome and transcriptome sequencing of Asian green mussel, Perna viridis
