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MicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting CPT1A and ACSL4

ABSTRACT: MicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting CPT1A and ACSL4

PROVIDER: PRJNA1226139 | ENA |

REPOSITORIES: ENA

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MicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting CPT1A and ACSL4

Project description:Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide, with limited effective treatment options. Recent studies highlight the emerging role of microRNAs in HCC biology, including microRNA-372-3p (miR-372-3p), but its precise function in HCC remains controversial. Here, we investigated the role of miR-372-3p in HCC using a transcriptomic approach. Overexpression of miR-372-3p in HCC cell lines significantly impaired proliferation, migration, invasion, and colony formation, indicating a tumor-suppressive function. RNA sequencing and Gene Set Enrichment Analysis (GSEA) revealed downregulation of genes involved in fatty acid metabolism, specifically fatty acid oxidation (FAO). Consistently, miR-372-3p overexpression increased lipid droplet accumulation and triglyceride levels while inhibiting FAO, leading to impaired lipid utilization under glucose deprivation and compromised interactions of lipid droplets with mitochondria and lysosomes. Mechanistically, we identified CPT1A and ACSL4 as direct targets of miR-372-3p using bioinformatics and dual luciferase assays. These findings demonstrate that miR-372-3p acts as a tumor suppressor in HCC by inhibiting FAO and disrupting lipid metabolism, suggesting a potential therapeutic target for HCC treatment.

2025-09-11 | GSE290065 | GEO

RNA-seq data in cardiac tissues from cardiomyocyte-specific Acsl4 knockout mice after transverse aortic constriction

Project description:In order to investigate the differentially expressed genes in Acsl4 knockout after TAC (Transverse aortic constriction) or sham surgery. The experiment was divided to four groups including sham-operated Acsl4 flox/flox mice (FS), sham-operated Acsl4 knockout mice (KS), TAC-operated Acsl4 flox/flox mice (FT), TAC-operated Acsl4 knockout mice (KT) n=3 mice/group. The heart tissue was isolated after 21 days after performing TAC or sham surgery.

2024-08-26 | E-MTAB-14383 | biostudies-arrayexpress

Bradyrhizobium sp. USDA 372 isolate:USDA 372

Project description:Bradyrhizobium sp. USDA 372 isolate:USDA 372 Genome sequencing

| PRJNA1142986 | ENA

The effect of ACSL4 expression on overall gene expression in breast cancer cell lines

Project description:ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and positively correlated with an aggressive breast cancer phenotype. Neither MCF-7 nor SKBr3 cells normally express ACSL4, and when manipulated to do so, develop basal-like characteristics, including increased proliferation, migration and anchorage independent growth. We used an Affymetrix array platform to assess changes in individual gene expression as a function of conditional and stable expression of ACSL4 in MCF-7 and SKBr3 cells. In experiment 1, MCF-7 Tet-ON cells were transfected with ACSL4 cDNA and induced to express ACSL4 by addition of 1ug/ml of doxycycline (A) or vehicle (C) for 72 hours. Three controls (C) and three doxycycline-induced (A) samples were evaluated for gene expression. In experiment 2, MCF-7 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A). In experiment 3, SKBr3 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A).

2012-09-19 | E-GEOD-40968 | biostudies-arrayexpress

miRNA changes after overexpression of uc.372 in HepG2 cells

Project description:To identify putative miRNA targets for uc.372, we overexpressed uc.372 in HepG2 cells and carried out a microarray analysis. A total of 66 miRNAs present in the array demonstrated 1.5-fold upregulation or downregulation of expression upon uc.372 transfection.

2017-11-01 | GSE106366 | GEO

ACSL4 effect on renal ischemia-reperfusion (I/R) injury

Project description:Analysis of acute kidney injury (AKI) with knockdown of ACSL4. Results provide insight into the role of ACSL4 in the regulation of ferroptosis.

2022-02-23 | GSE181842 | GEO

Sinorhizobium meliloti strain:372

Project description:Sinorhizobium meliloti 372 Resequencing

| PRJNA501531 | ENA

miR-101a-3p impairs synaptic plasticity and contributes to synucleinopathy

Project description:miR-101a-3p impairs synaptic plasticity and contributes to synucleinopathy

| PRJNA803028 | ENA

Effects and Mechanisms of ACSL4 Induces Immunosuppression in Colorectal Cancer

Project description:Analysis of knockout ACSL4 gene in mouse colorectal cancer cell line. These results are for the role of the ACSL4 gene in colorectal cancer.

2024-12-31 | GSE283772 | GEO

Effects and Mechanisms of ACSL4 Induces Immunosuppression in Colorectal Cancer [T84 cells]

Project description:Analysis of ACSL4 gene knockout in human colorectal cancer cell line. These results confirm the role of the ACSL4 gene in colorectal cancer.

2024-12-31 | GSE284613 | GEO

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