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Macrophages direct location-dependent recall of B cell memory to vaccination [scRNA-seq and scVDJ-seq]

ABSTRACT: Macrophages direct location-dependent recall of B cell memory to vaccination [scRNA-seq and scVDJ-seq]

PROVIDER: PRJNA1235184 | ENA |

REPOSITORIES: ENA

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Macrophages direct location-dependent recall of B cell memory to vaccination [scRNA-seq and scVDJ-seq]

Project description:Vaccines generate long-lived plasma cells and memory B cells (Bmems) that may re-enter secondary germinal centers (GCs) to further mutate their B cell receptor upon boosting and re-exposure to antigen. We show that lymph nodes draining the primary vaccination site harbour a subset of Bmems that reside in the subcapsular niche, generate larger recall responses, and are more likely to re-enter GCs compared to circulating Bmems in non-draining lymph nodes. Depletion of CD169+ subcapsular sinus macrophages impairs Bmem proliferative expansion, secondary GC formation and secretion of affinity-matured antibodies upon boosting of the draining lymph node. This site-specific, niche-dependent control is associated with more rapid secretion of broadly neutralizing antibodies, GC participation and clonal expansion of SARS-CoV-2-specific B cells in healthy volunteers boosted with the BNT162b2 vaccine in the same versus opposite arm. These data reveal an unappreciated role for primed draining lymph node SSMs in Bmem cell fate determination.

2025-06-30 | GSE291745 | GEO

Macrophages direct location-dependent recall of B cell memory to vaccination [CITE-seq]

Project description:Macrophages direct location-dependent recall of B cell memory to vaccination [CITE-seq]

| PRJNA1232810 | ENA

Macrophages direct location-dependent recall of B cell memory to vaccination [CITE-seq]

2025-06-30 | GSE291315 | GEO

Homo sapiens PBMCs: pre-vaccination (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination (stimulation vs. no stimulation with EV71 antigen)

Project description:To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. Four-condition experiment,pre-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen)

2015-09-03 | E-GEOD-72618 | biostudies-arrayexpress

CMV-CTLs scVDJ-RNAseq

Project description:CMV-CTLs scVDJ-RNAseq

| PRJNA659230 | ENA

Homo sapiens PBMCs: pre-vaccination (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination (stimulation vs. no stimulation with EV71 antigen)

Project description:To characterize the primary and recall responses to EV71 vaccines, PBMCs from 19 recipients before and after vaccination with EV71 vaccine were collected. 14 samples pre-vaccination and 16 samples post-vaccination were detected by microarray and their gene expression signatures after stimulation with EV71 antigen were compared.

2015-09-03 | GSE72618 | GEO

Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2 [scVDJ-seq]

Project description:Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2 [scVDJ-seq]

| PRJNA830586 | ENA

Pneumococcal conjugate vaccination at birth induces robust recall responses at 9 months of age.

Project description:The objective of this study was to compare recall responses to vaccine antigens at 3 months and 9 months of age in infants who were vaccinated at birth or at 1 month. PBMC were obtained at 9 months of age from infants who were vaccinated at birth (n=25) or 1 month (n=25). The PBMC were cultured in the presence or absence of CRM197 antigen for 72 hours. At the termination of the cultures, total RNA was extracted from the PBMC. The RNA was pooled into groups of n=5 subjects per group, following by labeling and hybridization to Affymetrix microarrays.

2011-06-27 | E-GEOD-25263 | biostudies-arrayexpress

Pneumococcal conjugate vaccination at birth induces robust recall responses at 9 months of age.

Project description:The objective of this study was to compare recall responses to vaccine antigens at 3 months and 9 months of age in infants who were vaccinated at birth or at 1 month.

2011-06-27 | GSE25263 | GEO

Multidimensional epigenomic priming of inflammatory genes enables rapid recall in human memory T cells

Project description:During T cell responses, a fraction of activated cells adopts a memory phenotype and returns to quiescence. These long-lived memory T cells retain an irreversible molecular imprint that enables them to mount a secondary recall response to the same antigen that is faster and greater in magnitude than the primary response of a naive cell. Memory T cells provide long-lasting immunological protection, forming the foundation for vaccination strategies and representing prime targets for immunotherapies to treat diseases characterized by dysfunctional memory T cells - including cancer and chronic inflammation. The molecular circuitry that endows memory T cells with their rapid recall ability remains incompletely understood. Here, we applied a multi-layered 1D-3D epigenomics approach to systematically dissect the molecular program driving rapid recall in primary human Th2 cells.

2023-07-24 | GSE207738 | GEO

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