Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1244055 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR32915953_1.fastq.gz | Fastqsanger.gz | |||
| SRR32915953_2.fastq.gz | Fastqsanger.gz | |||
| SRR32915954_1.fastq.gz | Fastqsanger.gz | |||
| SRR32915954_2.fastq.gz | Fastqsanger.gz | |||
| SRR32915955_1.fastq.gz | Fastqsanger.gz |
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2021-05-20 | GSE174713 | GEO
Project description:Ocular pigment epithelium (PE) cells promote the generation of T regulators (PE-induced Treg cells). Moreover, T cells exposed to PE acquire the capacity to suppress the activation of bystander T cells via TGFbeta. Membrane-bound TGFbeta on iris PE cells interacts with TGFbeta receptors on T cells, leading to the conversion of T cells to CD8(+) Treg cells via a cell contact-dependent mechanism. Conversely, soluble forms of TGFbeta produced by retinal PE cells can convert CD4(+) T cells into Treg cells in a manner that is independent of cell contact. In this study, we looked at the expression of immunoregulatory factors (TGFbeta, thrombospondins, CD59, IL-1 receptor antagonist, etc.) in PE cells as identified via an oligonucleotide microarray. Several thrombospondin-binding molecules were detected, and thus we focused subsequent analyses on thrombospondins. Via the conversion of latent TGFbeta to an active form that appears to be mediated by thrombospondin 1 (TSP-1), cultured iris PE and retinal PE cells induce a PE-induced Treg cell fate. After conversion, both ocular PE and PE-induced Treg cells express TSP-1. Regulatory T cell generation was amplified when the T cells also expressed TSP-1. In addition, PE-induced Treg cells significantly suppressed activation of bystander T cells via TSP-1. These results strongly suggest that the ability of ocular PE and PE-induced Treg cells to suppress bystander T cells depends on their capacity to produce TSP-1. Thus, intraocular TSP-1 produced by both ocular parenchymal cells and regulatory T cells is essential for immune regulation in the eye. Experiment Overall Design: Comparison of gene expression pattern in Mouse Primary Cultured Cell Lines (RPE, IPE, CBPE). Experiment Overall Design: Adult (6- to 8-week-old) C57BL/6 purchased from CLEA Japan Inc. (Tokyo, Japan) were used as donors ocular pigment epithelium (PE). To cultivate iris PE (IPE) and ciliary body PE (CBPE), iris and ciliary body tissues in the eyes were dissected out, and then incubated in 1 mg/ml Dispase and 0.05 mg/ml DNase I (both from Boehringer-Mannheim, Mannheim, Germany) for 1 hr. To cultivate retinal PE (RPE) Eyes were cut into two parts along a circumferential line posterior to the ciliary process, creating a ciliary body-deficient posterior eyecup. The eyecup was then incubated in 0.2% trypsin (Biowhitaker) for 1 hr. These tissues were triturated to make a single cell suspension, and then re-suspended in the medium.
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Project description:Apple is a woody tree in the Rosaceae family, genus Apple. It has been widely reported that MYBs are critical regulators for red color of apple peel by activating the expression of anthocyanin biogenesis genes. However, it is still not clear what is the molecular mechanism for the yellow color of apple peel. In order to investigate key genes and metabolites responsible for yellow coloration of apple peel, three strains of apples, "Venus Gold (Ype)" with yellow peel, "Yanfu8 (Mpe)" with medium red peel, and "Red love" with dark red peel, were selected in this study. Transcriptomic and metabolomic profiles were obtained for the peels of the three apple strains, respectively. After analyzing the transcriptomic profiles, our results suggest that DFR and LAR1 are two critical genes for the yellow color of Ype peel. Analysis of metabolomic profiles revealed that the abundances of Catechin and Epicatechin in Ype peel was higher than that of Rpe, indicating an important reason for the yellow color of Ype peel. Furthermore, when comparing volatile metabolites from Ype, Mpe and Rpe, hundreds of volatile metabolites show significantly differently abundances, suggesting that apples with different peel colors have different odors. In summary, our results provide new insights into the yellow coloration for the peels of some apple cultivars.
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