Project description:This study investigates the role of Ywhaz in early embryo development using a CRISPR-Cas9 knockout mouse model. RNA-seq was performed on pooled blastocysts derived from control and Ywhaz-knockout zygotes. Differential gene expression and pathway enrichment analysis revealed dysregulation of redox and cell proliferation pathways, providing insights into the molecular function of YWHAZ during preimplantation development.
Project description:Mouse embryos at the 2-cell to 8-cell stages were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. Subsequently, embryos were split into single blastomeres, and nuclei were isolated from each blastomere using a micromanipulator. Proteomic samples were then prepared using the in situ digestion of alcohol-fixed cells (iSDAC) method with 50 (2-cell), 216 (4-cell), and 280 (8-cell) nuclei per sample. The nuclear proteome during mouse preimplantation development was identified by label-free quantification.
Project description:Mouse Wdr74-knockout embryos were generated using the CRISPR-Cas9 system. Embryos from the 4-cell stage to the morula stage were collected and treated with acidic Tyrode's solution to remove the zona pellucida. We used 50 embryos per sample at each developmental stage and identified the proteome of mouse Wdr74-knockout embryos by label-free quantification.
Project description:Landmark events occur in a coordinated manner during preimplantation development of the mammalian embryo, yet the regulatory network that orchestrates these events remains largely unknown. We delineated the regulons of Oct4, Sall4, and Nanog using morpholino (MO)-mediated gene knockdowns followed by microarray analysis of pooled embryos.
Project description:Purpose: The goals of this study are to define transcriptome (RNA-seq) of mouse preimplantation embryos at different stages of development under a range of different environmental conditions. Methods: Mouse preimplantation embro transcriptional profiles were generated using embryos at several different developmental stages using Smart-seq2. Results: RNA-seq analysis finds that there is a highly dynamic pattern of gene expression during the preimplantation period. The sensitiivty to nutrient conditions varies markedly at different stages of development, with 2C embryos more sensitive to pyruvate omission than later stage embryos. Conclusions: Our study represents a comprehensive analysis of the mouse preimplantation development transcriptome, and how pyruvate provision impacts different developmental stages.
Project description:We investigated how RIF1 depletion affects the nuclear organization in mouse preimplantation embryos by performing DamID-seq to map lamina-associated domains (LADs).
Project description:Landmark events occur in a coordinated manner during preimplantation development of the mammalian embryo, yet the regulatory network that orchestrates these events remains largely unknown. We delineated the regulons of Oct4, Sall4, and Nanog using morpholino (MO)-mediated gene knockdowns followed by microarray analysis of pooled embryos. 1-cell fertilized mouse embryos were injected with a morpholino and were collected at either 20 hours or at 44 hours after microinjection.