Project description:The genus Bothrops is responsible for most part of envenomation accidents in Brazil. Bothrops pubescens is an endemic and neglected species in the Brazilian Pampa Biome. The characterization of its venom is essential since there is no data about it and can be helpful in the discovery of active biomolecules and for a better understanding of its action. We used top-down (TDP), native top-down, and bottom-up proteomic (BUP) approaches to characterize the venom of B. pubescens. We were able to identify 89 protein groups with the BUP approach and 40 unique proteoforms with the TDP approach, demonstrating the similarities and peculiarities of B. pubescens venom. We also identified a dimeric L-amino acid oxidase with using native TDP. Here we present for the first time a bothropic venom characterization through TDP approaches.
Project description:RNA sequencing of Apis mellifera abdominal fat body and matched whole brain following a knockdown in fat body ame-miR-305-5p expression
Project description:We analyzed the changes in the brain tissue of Apis mellifera ligustica at the molecular level by sequencing after using fluvalinate. We found that the differentially expressed miRNAs (DEM) may be involved in hippocampal cell apoptosis and damage to memory functions. This result may be related to behaviors observed after the administration of this medication, such as a lack of homing at night and behavioral disturbances. Overall, our results provide new information about the molecular mechanisms and pathways of fluvalinate action in the brain tissue of Apis mellifera ligustica.
Project description:The 5’ LongSAGE (5’LS) approach provides a powerful genomic tool for identifying Transcription start sites (TSSs) in sequenced genome. The main purpose of this study is to identify the actual TSSs of expressed genes as well as the usage of different TSSs in Apis mellifera. We also wish to provide expression evidence for the in silico predicted genes and reveal some previously undiscovered genes.
Project description:We have identified a honeybee (Apis mellifera) odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA) from four candidate sex pheromone odorant receptors from the honeybee genome based on their biased expression in drone antennae. Keywords: Tissue Comparison
Project description:The central part of Brazil, consisting mostly of the Cerrado Biome, is considered to be the new frontier for increasing Brazilian wheat production. However, rainfed wheat production in that area must cope with drought stress. In order to better understand the drought response, we analyzed the mRNA profiling under drought in roots and leaves of the cultivar MGS1 Aliança (a well-adapted cultivar to the Cerrado). We identified 4,422 candidate genes in roots and leaves.
Project description:To explore brain neuropeptidic functions in behavioral regulation, a label-free quantitative strategy was employed to compare neuropeptidomic variations between behavioral phenotypes (nurse bees, nectar foragers, and pollen foragers) and the two honeybee species (Apis mellifera ligustica and Apis cerana cerana).