Project description:This project presents the first proteome-wide overview of lysine crotonylation (Kcr) in Reynoutria japonica. A total of 18,914 Kcr sites were identified across 5,842 proteins, and six conserved motifs were characterized. The dataset provides a valuable reference for future epigenetic studies in medicinal plants and offers insights into post-translational regulation in R. japonica.
Project description:We created a triple loss-of-function/knockout mutant targeting three rice (Oryza sativa japonica) genes simultaneously via the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/Cas9 system. The three selected genes are as follows: OsPRK1 (LOC_Os08g40990), OsPRK2 (LOC_Os06g45240), and OsPRK3 ((LOC_Os02g07810). These three OsPRKs are strongly transcriptional expressed in the mature anthers of rice (stages 13) and bi-/tricelluler pollen. The triple mutant of these OsPRKs does not produce self-fertilized seeds due to defects in the hydration and germination of pollen grains (male-sterile). This data is about RNA-sequencing transcriptome data about the Oryza sativa Dongjin used as a control and triple mutant of OsPRKs (OsPRK1/2/3). We sampled mature anther for the analysis.
