Project description:Sparganosis is a neglected food- and water-borne zoonotic disease caused by members of the tapeworm genus Spirometra. More than 1600 human cases have been reported in the literature, with most originating mainly from Korea and China, but the clinical relevance of sparganosis is likely underestimated. Here, we employed LC-MS/MS to charaterise somatic proteome of Spirometra proliferum.
Project description:Background: Sparganosis caused by Spirometra erinaceieuropaei spargana is a zoonotic parasitic infection which has been reported in many countries, such as China, Japan, Thailand and Korea, as well as the Europe and United States. This parasite biological and clinical significances, genome, and transcriptome analysis have previously been investigated, but its phosphoproteomes have not been reported. Here, we investigated global and site-specific phosphoproteome profiling of the spargana. Results: 3228 phosphopeptides and 3461 phosphorylation sites were identified from 1758 spargana proteins. The annotated phosphoproteins were involved in the various biological mechanisms, which included the cellular, metabolic and single-organism processes. Additionally, functional enrichment of phosphopepetides in Gene Ontology analysis suggested that most spargana phosphoproteins were related to the cytoskeleton cellular compartment, the signaling molecular function, and a variety of biological processes, including a molecular function regulator, guanyl-nucleotide exchange factor activity, protein kinase activities, and calcium ion binding. The highly enriched pathway of phosphorylation proteins included the phosphatidylinositol signaling system, phagosome, endocytosis, inositol phosphate metabolism, terpenoid backbone biosynthesis, and peroxisome pathways. Domain analysis of phosphopeptides identified EF-hand domain and pleckstrin homology domain among the important domains. Conclusions: This study provides the first global phosphoproteomic analysis in the spargana. The reported dataset will shed light on future understanding of zoonotic parasite.
Project description:Genomic assembly of cestode Spirometra erinaceieuropaei, as part of the 50 Helminth Genomes Initiative sequencing of the parasitic worms that have the greatest impact on human, agricultural and veterinary disease and cause significant global health issues particularly in the developing world, or those used as model organisms.
