Project description:Background: Sparganosis caused by Spirometra erinaceieuropaei spargana is a zoonotic parasitic infection which has been reported in many countries, such as China, Japan, Thailand and Korea, as well as the Europe and United States. This parasite biological and clinical significances, genome, and transcriptome analysis have previously been investigated, but its phosphoproteomes have not been reported. Here, we investigated global and site-specific phosphoproteome profiling of the spargana. Results: 3228 phosphopeptides and 3461 phosphorylation sites were identified from 1758 spargana proteins. The annotated phosphoproteins were involved in the various biological mechanisms, which included the cellular, metabolic and single-organism processes. Additionally, functional enrichment of phosphopepetides in Gene Ontology analysis suggested that most spargana phosphoproteins were related to the cytoskeleton cellular compartment, the signaling molecular function, and a variety of biological processes, including a molecular function regulator, guanyl-nucleotide exchange factor activity, protein kinase activities, and calcium ion binding. The highly enriched pathway of phosphorylation proteins included the phosphatidylinositol signaling system, phagosome, endocytosis, inositol phosphate metabolism, terpenoid backbone biosynthesis, and peroxisome pathways. Domain analysis of phosphopeptides identified EF-hand domain and pleckstrin homology domain among the important domains. Conclusions: This study provides the first global phosphoproteomic analysis in the spargana. The reported dataset will shed light on future understanding of zoonotic parasite.
Project description:Genomic assembly of cestode Spirometra erinaceieuropaei, as part of the 50 Helminth Genomes Initiative sequencing of the parasitic worms that have the greatest impact on human, agricultural and veterinary disease and cause significant global health issues particularly in the developing world, or those used as model organisms.
Project description:This study was carried out to provide information on the taxonomic classification and analysis of mitochondrial genomes of Spirometra theileri. One strobila of S. theileri was collected from the intestine of an African leopard (Panthera pardus) in the Maswa Game Reserve, Tanzania. The complete mtDNA sequence of S. theileri was 13,685 bp encoding 36 genes including 12 protein genes, 22 tRNAs and 2 rRNAs with absence of atp8. Divergences of 12 protein-coding genes were as follow: 14.9% between S. theileri and S. erinaceieuropaei, 14.7% between S. theileri and S. decipiens, and 14.5% between S. theileri with S. ranarum. Divergences of 12 proteins of S. theileri and S. erinaceieuropaei ranged from 2.3% in cox1 to 15.7% in nad5, while S. theileri varied from S. decipiens and S. ranarum by 1.3% in cox1 to 15.7% in nad3. Phylogenetic relationship of S. theileri with eucestodes inferred using the maximum likelihood and Bayesian inferences exhibited identical tree topologies. A clade composed of S. decipiens and S. ranarum formed a sister species to S. erinaceieuropaei, and S. theileri formed a sister species to all species in this clade. Within the diphyllobothridean clade, Dibothriocephalus, Diphyllobothrium and Spirometra formed a monophyletic group, and sister genera were well supported.