Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA127655 | ENA |
REPOSITORIES: ENA
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Project description:Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis. Toluidine blue-stained cryostat sections of embryonic day 12.5 submandibular salivary glands were used for laser microdissection using an LMD system (Leica) with a pulsed UV laser on an upright automated microscope. A total of approximately 500 cells from a region immediately adjacent to a forming cleft or from a peripheral end bud region were excised by the laser and transferred to caps of 0.5 ml PCR tubes. SAGE libraries were constructed from the microdissected cleft and bud epithelia cells. SAGE tags were identified using the National Center for Biotechnology Information (NCBI) tag map set for UniGene.
2010-06-16 | E-GEOD-22374 | biostudies-arrayexpress
Project description:Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis.
2010-06-17 | GSE22374 | GEO
Project description:Our single-cell and bulk transcriptome analyses revealed striking molecular heterogeneity in the mesenchymal cells of embryonic submandibular and parotid salivary glands during bud initiation, including distinct mesodermal and neural crest-derived molecular signatures that foreshadow later functions.
2019-10-25 | GSE127260 | GEO
Project description:Our single-cell and bulk transcriptome analyses revealed striking molecular heterogeneity in the mesenchymal cells of embryonic submandibular and parotid salivary glands during bud initiation, including distinct mesodermal and neural crest-derived molecular signatures that foreshadow later functions.
2019-10-25 | GSE127469 | GEO
Project description:The circumvallate papillae (CVP) and foliate papillae (FoP) of the posterior tongue contain taste buds in close proximity to specialized salivary glands, known as von Ebner and minor salivary glands, respectively. The developmental relationship between taste buds and these salivary glands remains largely unexplored. Lineage tracing studies in mice have revealed that Lgr5 marks taste bud stem cells. Here, we report single-cell RNA sequencing of the entire CVP and FoP of mice, yielding transcriptional profiles of cells from tongue surface epithelium, taste buds and the associated salivary glands. We unveiled a developmental trajectory in which taste buds, the associated salivary glands and the non-taste tongue surface epithelium originate from a common Lgr5+ cell. We describe long-term organoid culture conditions for these cells and confirm their tripotency at the clonal level in vitro. CVP and FoP harbor chemosensory units consisting of taste bud and salivary gland cells derived from the same parental Lgr5+ stem cell.
2025-09-01 | GSE274015 | GEO
Project description:The circumvallate papillae (CVP) and foliate papillae (FoP) of the posterior tongue contain taste buds in close proximity to specialized salivary glands, known as von Ebner and minor salivary glands, respectively. The developmental relationship between taste buds and these salivary glands remains largely unexplored. Lineage tracing studies in mice have revealed that Lgr5 marks taste bud stem cells. Here, we report single-cell RNA sequencing of the entire CVP and FoP of mice, yielding transcriptional profiles of cells from tongue surface epithelium, taste buds and the associated salivary glands. We unveiled a developmental trajectory in which taste buds, the associated salivary glands and the non-taste tongue surface epithelium originate from a common Lgr5+ cell. We describe long-term organoid culture conditions for these cells and confirm their tripotency at the clonal level in vitro. CVP and FoP harbor chemosensory units consisting of taste bud and salivary gland cells derived from the same parental Lgr5+ stem cell.
2025-09-01 | GSE274014 | GEO
Project description:Comparisons between the salivary glands upon the RNAi of sage gene vs. the control. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other
2003-07-23 | GSE283 | GEO
Project description:Comparisons between the salivary glands upon the RNAi of sage gene vs. the control. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development
2010-06-05 | E-GEOD-283 | biostudies-arrayexpress
Project description:Salivary glands are essential structures that secrete saliva to the oral cavity and maintain oral health. Development of salivary glands in mice and humans is controlled by mesenchymally expressed fibroblast growth factor-10 (FGF10). Using single cell RNA-seq atlas of the salivary gland and a tamoxifen inducible Fgf10CreERT2:R26-tdTomato mouse we show that FGF10pos cells are exclusively mesenchymal until postnatal day 5 (P5), but after P7, there is a switch in expression and only epithelial FGF10pos cells are observed after P15. Further RNAseq analysis of sorted mesenchymal and epithelial FGF10pos cells shows that the epithelial FGF10pos populations express the hallmark of ancient ionocyte signature Foxi1, Foxi2, Ascl3 and the cystic fibrosis transmembrane conductance regulator (Cftr). We propose that epithelial FGF10pos cells are specialized salivary gland ionocytes that are important for the ionic modification of saliva. In addition, they maintain FGF10-dependent glands homeostasis via communication with FGFR2b-expressing epithelial progenitor and myoepithelial cells
2022-04-12 | GSE188904 | GEO
Project description:Branching morphogenesis is a basic way of the kinds of complex organs’ development including lungs, kidneys, thyroid glands and salivary glands.1 Submandibular gland (SMG) development is a classic model to reveal the mechanism of branching morphogenesis and it also provides novel approaches to tissue engineering for salivary glands regenerating or for creating artificial salivary glands.And for murine salivary glands’ development, two main components are formed, epithelial and mesenchyme. The elongation and branch of epithelial make up the main process of its development which supported by mesenchymal secretion.This processes are controlled by many factors like growth factors, hormones and miRNAs etc.567 All these factors play different roles in the branching morphogenesis of mouse salivary glands. Transforming growth factor β1 (TGF-β1) is a pivotal factor of them and it has a huge impact on SMG development through its action on the mesenchyme. We used microarrays to detail the global programme of gene expression during the branching morphogenesis and identified distinct classes of up/down-regulated genes during this process.
2019-08-21 | GSE136061 | GEO