ABSTRACT: Caenorhabditis elegans Raw sequence reads-Evaluation of SCCPs which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of DP which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
| PRJNA1283975 | ENA
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of 6-PPDQ which can induce germ-cell mutagenesis in alternative in vivo model C.elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of chemotherapeutic agent oxaliplatin-induced germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of chemotherapeutic agent Flame retardant of TBBPA, TCEP, TCPP which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Raw sequence reads Evaluation of chemotherapeutic agent oxaliplatin-induced germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of heavy metal of potassium dichromate, cadmium chloride, sodium arsenite which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:This dataset comprises RNA sequencing and downstream functional analyses of Caenorhabditis elegans models of spinal muscular atrophy (SMA). Samples were collected at the early L4 larval stage from wildtype and smn-1 mutant strains to assess gene expression changes associated with SMA phenotype onset. The submitted data include processed tables for gene ontology enrichment, alternative splicing events, and differential gene expression analyses performed to characterize molecular pathways impacted by smn-1 deficiency. Raw reads for all samples are available in ENA under the provided accession numbers. This dataset supports the findings published in the corresponding manuscript describing SMA pathomechanisms using C. elegans as a model organism.
Project description:The Cell Division Cycle and Apoptosis Regulator (CCAR) protein family members have recently emerged as regulators of alternative splicing and transcription, as well as having other key physiological functions. For example, mammalian CCAR2/DBC1 forms a complex with the zinc factor protein ZNF326 to integrate alternative splicing with RNA polymerase II transcriptional elongation in AT-rich regions of the DNA. Additionally, Caenorhabditis elegans CCAR-1, a homolog to mammalian CCAR2, facilitates the alternative splicing of the perlecan unc-52 gene. However, much about the CCAR family's role in alternative splicing is unknown. We are interested in uncovering the role of the CCAR family in alternative splicing in vivo using Caenorhabditis elegans. We examined the role of CCAR-1 in genome-wide alternative splicing and identified new alternative splicing targets of CCAR-1 using RNA sequencing. Also, we found that CCAR-1 interacts with the spliceosome factors UAF-1 and UAF-2 using mass spectrometry, and that knockdown of ccar-1 affects alternative splicing patterns, motility, and proteostasis of UAF-1 mutant worms. Collectively, we demonstrate a role for CCAR-1 in the regulation of global alternative splicing in C. elegans and in conjunction with UAF-1
Project description:we used Caenorhabditis elegans as a model organism, to investigate the effect of mannose on the lifespan. Using nematode RNAi methods, RT-PCR, RNA-seq and other experimental method, we explored the possible mechanism for how mannose change the lifespan of Caenorhabditis elegans.