Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of SCCPs which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
| PRJNA1283963 | ENA
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of DP which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of chemotherapeutic agent oxaliplatin-induced germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of chemotherapeutic agent Flame retardant of TBBPA, TCEP, TCPP which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Raw sequence reads Evaluation of chemotherapeutic agent oxaliplatin-induced germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:Caenorhabditis elegans Raw sequence reads-Evaluation of heavy metal of potassium dichromate, cadmium chloride, sodium arsenite which can induce germ-cell mutagenesis in alternative in vivo model Caenorhabditis elegans
Project description:We have extended our array-CGH analysis of genetic deficiencies in Caenorhabditis elegans to a set on LGV (left) balanced by the reciprocal translocation eT1. This set includes 20 deletions and a single duplication. Keywords: C.elegans Deficiencies CGH
Project description:To examine the protein composition of germ granules subcompartments of C. elegans, while ensuring their physiological integrity, we utilized TurboID-based proximity biotin labeling techniques to idedtify the protein components of P granules, Z granules and Mutator foci in the germline of C.elegans.
Project description:The Cell Division Cycle and Apoptosis Regulator (CCAR) protein family members have recently emerged as regulators of alternative splicing and transcription, as well as having other key physiological functions. For example, mammalian CCAR2/DBC1 forms a complex with the zinc factor protein ZNF326 to integrate alternative splicing with RNA polymerase II transcriptional elongation in AT-rich regions of the DNA. Additionally, Caenorhabditis elegans CCAR-1, a homolog to mammalian CCAR2, facilitates the alternative splicing of the perlecan unc-52 gene. However, much about the CCAR family's role in alternative splicing is unknown. We are interested in uncovering the role of the CCAR family in alternative splicing in vivo using Caenorhabditis elegans. We examined the role of CCAR-1 in genome-wide alternative splicing and identified new alternative splicing targets of CCAR-1 using RNA sequencing. Also, we found that CCAR-1 interacts with the spliceosome factors UAF-1 and UAF-2 using mass spectrometry, and that knockdown of ccar-1 affects alternative splicing patterns, motility, and proteostasis of UAF-1 mutant worms. Collectively, we demonstrate a role for CCAR-1 in the regulation of global alternative splicing in C. elegans and in conjunction with UAF-1
