Project description:RNA-seq profiling of Tsc2-deficient mouse embryonic fibroblasts (MEFs) and TSC2-deficient 621-101 human angiomyolipoma cells exposed to oleic acid or basal condition.
Project description:Compared to ordinary rapeseed, high-oleic acid rapeseed has higher levels of monounsaturated fatty acids and lower levels of saturated fatty acid and polyunsaturated fatty acids, and thus is of high nutritional and health value. In addition, high-oleic acid rapeseed oil imparts cardiovascular protective effects. Based on these properties, high-oleic acid oil crops have been extensively investigated and cultivated. In this study, we employed a microarray analysis with high oleic acid line and low oleic acid line from the developing seeds (27 days after flowering) of Brassica napus.
Project description:S. aureus response to exogenous fatty acid (oleic acid) Gene expression profiles were generated by microarray analysis of S. aureus cells grown in media without or with oleic aicd Comparison of expression profiles after growth of S. aureus in exogenous fatty acid S. aureus was grown in media with and without oleic acid to an OD600nm of 0.5, and RNA was extracted to look at the gobal gene expression.
Project description:Iron overload, characterized by accumulation of iron in tissues, induces a multiorgan toxicity whose mechanisms are not fully understood. Using cultured cell lines, Caenorhabditis elegans, and mice, we found that ferroptosis occurs in the context of iron-overload-mediated damage. Exogenous oleic acid protected against iron-overload-toxicity in cell culture and Caenorhabditis elegans by suppressing ferroptosis. In mice, oleic acid protected against FAC-induced liver lipid peroxidation and damage. Oleic acid changed the cellular lipid composition, characterized by decreased levels of polyunsaturated fatty acyl phospholipids and decreased levels of ether-linked phospholipids. The protective effect of oleic acid in cells was attenuated by GW6471 (a PPAR- antagonist), as well as in Caenorhabditis elegans lacking the nuclear hormone receptor NHR-49 (a PPAR- functional homologue). These results highlight ferroptosis as a driver of iron-overload-mediated damage, which is inhibited by oleic acid. This monounsaturated fatty acid represents a potential therapeutic approach to mitigating organ damage in iron overload individuals.
Project description:S. aureus response to exogenous fatty acid (oleic acid) Gene expression profiles were generated by microarray analysis of S. aureus cells grown in media without or with oleic aicd
Project description:T cells are the most common immune cell in atherosclerotic plaques. Furthermore, T cell function is influenced by fatty acids that are abundant in the circulation and atherosclerotic plaques. Here, we define the effect of oleic acid, one of the most abundant fatty acids in the human circulation, on the transcriptome of resting CD4+ T cells and subsequently the polarization of the cells into T cell subsets post-activation. We first performed RNA sequencing of resting CD4+ T cells from 9 donors after 0.5, 3, 24, 48, and 72 hours exposure to 30µg/mL oleic acid. This revealed the upregulation of genes involved in cholesterol biosynthesis (HMGCR, SQLE, MVD and MVK) and de novo fatty acid biosynthesis (ACACA and FASN). Both pathways point to a metabolic reprogramming of the cells towards a pro-inflammatory state. Subsequently, a 26-marker spectral flow cytometry panel showed increased frequencies of IL-9+ and IL-17A+ cells among activated CD4+ T cells pre-exposed to oleic acid. IL-9 and IL-17A are primarily produced by the respective T helper (TH) subsets TH9 and TH17, where, especially TH9, has been implicated in the aggravation of atherosclerosis. Our data shows that oleic acid may induce a shift in immunometabolism in resting CD4+ T cells towards an activated profile that leads to more pro-inflammatory and pro-atherogenic polarization of these cells post-activation. Taken together, our study signals a potential role for the interaction between circulating fatty acids and CD4+ T cells in the development and pathogenesis of atherosclerosis.
Project description:T cells are the most common immune cell in atherosclerotic plaques. Furthermore, T cell function is influenced by fatty acids that are abundant in the circulation and atherosclerotic plaques. Here, we define the effect of oleic acid, one of the most abundant fatty acids in the human circulation, on the transcriptome of resting CD4+ T cells and subsequently the polarization of the cells into T cell subsets post-activation. We first performed RNA sequencing of resting CD4+ T cells from 9 donors after 0.5, 3, 24, 48, and 72 hours exposure to 30µg/mL oleic acid. This revealed the upregulation of genes involved in cholesterol biosynthesis (HMGCR, SQLE, MVD and MVK) and de novo fatty acid biosynthesis (ACACA and FASN). Both pathways point to a metabolic reprogramming of the cells towards a pro-inflammatory state. Subsequently, a 26-marker spectral flow cytometry panel showed increased frequencies of IL-9+ and IL-17A+ cells among activated CD4+ T cells pre-exposed to oleic acid. IL-9 and IL-17A are primarily produced by the respective T helper (TH) subsets TH9 and TH17, where, especially TH9, has been implicated in the aggravation of atherosclerosis. Our data shows that oleic acid may induce a shift in immunometabolism in resting CD4+ T cells towards an activated profile that leads to more pro-inflammatory and pro-atherogenic polarization of these cells post-activation. Taken together, our study signals a potential role for the interaction between circulating fatty acids and CD4+ T cells in the development and pathogenesis of atherosclerosis.
Project description:Lymphangioleiomyomatosis (LAM) is characterized by cystic lung destruction caused by smooth, muscle-like LAM cells which have mutations in the tumor suppressor genes Tuberous Sclerosis Complex (TSC) 1 or 2, and the capacity to metastasize. Since chemokines and their receptors function in chemotaxis of metastatic cells, we hypothesized that LAM cells may be recruited by chemokine(s) in the lung. Quantification of 25 chemokines in bronchoalveolar lavage fluid from LAM patients and healthy volunteers revealed that concentrations of MCP-1/CCL2, GROa/CXCL1 and ENA-78/CXCL5 were significantly higher in samples from LAM patients than healthy volunteers. In this transcript analysis, expression of chemokine and chemokine receptor mRNA in LAM cells differed from those in melanoma and smooth muscle cells. Subsequent immunohistochemistry of lung sections from 30 LAM patients confirmed protein expression of chemokines and these receptors varied among LAM patient and differed from that seen in breast cancer and melanoma cells. . In vitro, MCP-1/CCL2 induced selective migration of cells showing loss of heterozygosity of TSC2 from a heterogeneous populations of cells grown from explanted LAM lungs. In addition, the frequencies of single-nucleotide polymorphisms in the MCP-1 gene promoter region differed significantly in LAM patients and healthy volunteers (p=0.018), and one polymorphism was associated significantly more frequently with the decline of lung function. These observations are consistent with the notion that chemokines such as MCP-1 may serve to specify site of LAM cell metastasis. Keywords: Human patient sample comparison with cell lines The study is of case/control design with biological replication. Biopsies of nodules from 14 LAM patients (cases) are compared with cultured cell lines (controls) with similar properties.
Project description:To identify differentially expressed proteins in a high-oleic acid rapeseed line, self-bred seeds (20 to 35 days after pollination) of a high- and a low-oleic acid rapeseed near-isogenic line (oleic acid contents of 81.4% and 56.2%, respectively) were used as raw materials for iTRAQ (isobaric tags for relative and absolute quantitation) analysis.