Project description:Bud endodormancy induction response of two genotypes (Seyval, a hybrid white wine grape and Vitis riparia, PI588259, a native North American grape species) was compared under long (15 h) and short (13 h) photoperiods. Proteins were extracted from both genotypes for all time points and experimental conditions. The proteins were separaed by 2D-PAGE, trypsin digested, and the peptides identified with a MALDI-TOF-TOF mass spectrometer. A master gel was made and mapped with all proteins from both genotypes. The proteins were identified by matching the peptide sequences against the 8X Vitis vinifera grape genome in NCBI. This study was funded by NSF grant DBI064755 and is the result of a collaboration between Dr. Anne Fennell at South Dakota State University and Dr. Grant R. Cramer at the University of Nevada, Reno.
Project description:The aim of this quantitative label-free shotgun proteomic experiment is to perform a comparative study of two different sample preparation methods which employ two different pre-fractionation techniques (SDS-PAGE and FASP-GPF) for use in shotgun proteomic analysis of Vitis riparia leaf material with concurrent label-free quantitation in order to optimise a technique that would facilitate identification of most number of proteins and produce useful biological information, when searched against the Vitis vinifera database.
