Project description:We applied nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) in a single Trionyx triunguis reference specimen to identify diagnostic COl1 peptide markers. Our analysis revealed four COL1 biomarkers that differentiate T. triunguis from all sea turtle species, as well as seven additional turtle species with COL1 sequences available on NCBI: Chelonoidis abingdonii, Chrysemys picta bellii, Gopherus evgoodei, Mauremys reevesii, Pelodiscus sinensis, Terrapene carolina triunguis, and Trachemys scripta elegans. These findings enhance biomolecular identification methods for freshwater and marine turtle remains in archaeological and ecological contexts (especially on the Levant).
Project description:interdigital cell death (ICD) is an essential role in amniotes to promote the regression of interdigital tissue for digits separation. Antagonizing this process can lead to the formation of webbed limbs. However, the mechanism of forming webbed limbs is not the same among independently evolved species, and the reason is still unclear. The Chinese softshell turtle (Pelotiscus sinensis) is a good research model, exhibiting a transitional feature from no webbed to fully webbed. In this study, we collected 8 samples of interdigital webbing from the forelimbs and hindlimbs of turtles at embryonic stage 19 (TK19) for RNA sequencing. We identified 608 potential regulatory genes through differential expression analysis. These findings not only provide new perspectives and data support for comparative studies of adaptive convergent evolution in aquatic animals, but also enhance our understanding of the mechanisms underlying quadrupedal limb morphology. In addition, these results provide potential molecular targets for studying the plasticity of programmed cell death or aging.
2025-05-08 | GSE287055 | GEO
Project description:Intestinal microorganisms of Chinese softshell turtle
