Project description:Transcriptomic analysis of LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana Examination of differential gene expressions by Beauveria bassiana wild type, LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana
Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by pspA, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of pspA disruption and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10430 genes annotated or not annotated. Among those, 1818 genes (genomic 17.54%) were dysregulated in the absence of pspA, including 806 down-regulated and 1012 up-regulated. Conclusions: pspA lacking any predictable function domain has profound impact on the genomic expression of Beauveria bassiana.
Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by Cre1, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of cre1 deletion and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the deletion mutant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10364 genes annotated or not annotated. Among those, 1881 genes (genomic 18.149%) were dysregulated in the absence of cre1, including 1117 down-regulated and 764 up-regulated. Conclusions: Cre1 has profound impact on the genomic expression of Beauveria bassiana.
2021-10-02 | GSE184973 | GEO
Project description:Beauveria bassiana White Collar-1, White Collar-2 defective strains
Project description:Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of dim5 disruption and wild-type strains grown under normal culture conditions and and subjected to deep sequencing on Illumina Novaseq 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10915 genes annotated or not annotated. Among those, 1201 genes (genomic 11.59%) were dysregulated in the absence of dim5, including 489 down-regulated and 712 up-regulated. Conclusions: DIM5 has profound impact on the genomic expression of Beauveria bassiana.
Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Acetylomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.
Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Proteomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.
