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ABSTRACT: Beauveria bassiana White Collar-1, White Collar-2 defective strains

PROVIDER: PRJNA931465 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23339335_1.fastq.gz	Fastqsanger.gz
	SRR23339335_2.fastq.gz	Fastqsanger.gz
	SRR23339336_1.fastq.gz	Fastqsanger.gz
	SRR23339336_2.fastq.gz	Fastqsanger.gz
	SRR23339337_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of LaeA and LaeB deletion strains in fungus Beauveria bassiana

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Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by pspA, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of pspA disruption and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10430 genes annotated or not annotated. Among those, 1818 genes (genomic 17.54%) were dysregulated in the absence of pspA, including 806 down-regulated and 1012 up-regulated. Conclusions: pspA lacking any predictable function domain has profound impact on the genomic expression of Beauveria bassiana.

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Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by Cre1, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of cre1 deletion and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the deletion mutant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10364 genes annotated or not annotated. Among those, 1881 genes (genomic 18.149%) were dysregulated in the absence of cre1, including 1117 down-regulated and 764 up-regulated. Conclusions: Cre1 has profound impact on the genomic expression of Beauveria bassiana.

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