Project description:We are investigating the mRNA expression profiles of human lung cells to gaseous urban mixtures We used microarrays to compare the global mRNA expression profiles upon response to fresh against aged urban mix Keywords: dose A549 cells were grown to confluency and exposed to fresh urban mix, aged urban mix, or mock-treated. RNA was collected 9 hrs after exposure.
Project description:We are investigating the mRNA expression profiles of human lung cells to gaseous urban mixtures We used microarrays to compare the global mRNA expression profiles upon response to fresh against aged urban mix Keywords: dose
Project description:2D (A549) and 3D (AIR-606) human lung epithelial cell cultures were mock treated or exposed to 100 ug/ml petroleum coke or urban air particulates (both in the PM10 range) for 16 h. Secretomes were compared for triplicate treatments for each group by isobaric tags and LC/MS/MS.
Project description:To investigate the toxicological mechanisms induced by an antrhopogenic SOAs (SOANAP-SP) and a biogenic SOAs (SOAβPIN-SP), as well as, fresh soot particles (SPs) in either the directly exposed A549 cells cultured at the air-liquid interphase or in the indirectly exposed EA.hy926 cells.
Project description:In this study, fathead minnow (FHM) embryos were exposed to two doses of fresh, aged and pure naphthenic acids mixtures until hatch. Larvae were scored for developmental deformities prior to preservation for gene expression analyses. 5 larvae were pooled per gene expression replicate.
Project description:Human lung cells (BEAS-2B) were exposed to either liquid suspensions of 125 ug/mL urban particulate matter (NIST, SRM 1648a) or growth media only control for 24 hours at 37C, 5% CO2. We have explored the changes in expression level and subcellular abundance of key proteins involved in cellular response to air pollution stress.
Project description:This study compares the dose dependent transcriptome data obtained for the volatile compound dimethylamine, which has been exposed in an in vitro system via air-liquid-interface (ALI) exposure to a pulmonary cell line (A549 cells).
Project description:This study compares the dose dependent transcriptome data obtained for the volatile compound dimethylamine, which has been exposed in an in vitro system via air-liquid-interface (ALI) exposure to a pulmonary cell line (A549 cells).
Project description:This study compares the dose dependent transcriptome data obtained for the volatile compound dimethylamine, which has been exposed in an in vitro system via air-liquid-interface (ALI) exposure to a pulmonary cell line (A549 cells).
