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Bulk RNA-seq of RSC96 Schwann cells after magnetic nanoparticle uptake and magnetic force stimulation

ABSTRACT: Bulk RNA-seq of RSC96 Schwann cells after magnetic nanoparticle uptake and magnetic force stimulation

PROVIDER: PRJNA1308865 | ENA |

REPOSITORIES: ENA

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Bulk RNA-seq of RSC96 Schwann cells after magnetic nanoparticle uptake and magnetic force stimulation

Project description:To explore the effect of magnetic mechanical forces mediated by magnetic nanoparticles on the repair phenotype of Schwann cells in a gradient magnetic field environment, we co-cultured magnetic nanoparticles with RSC96 cells.

2025-08-24 | GSE305996 | GEO

Bulk RNA-seq of rat sciatic nerve distal to crush after magnetic nanoparticle–mediated magnetic force stimulation

Project description:To explore the effect of magnetic mechanical force mediated by magnetic nanoparticles in a gradient magnetic field environment on the repair phenotype of Schwann cells in injured sciatic nerves, we established a rat sciatic nerve crush injury model and locally injected magnetic nanoparticles under the epineurium at the distal end of the crush site.

2025-08-24 | GSE306137 | GEO

Bulk RNA-seq of rat sciatic nerve distal to crush after magnetic nanoparticle–mediated magnetic force stimulation

Project description:Bulk RNA-seq of rat sciatic nerve distal to crush after magnetic nanoparticle–mediated magnetic force stimulation

| PRJNA1309573 | ENA

MiRNA sequencing of Schwann-like cells induced by Schwann cell-derived exosomes

Project description:Background: Adipose-derived stem cells (ADSCs) can differentiate into Schwann cells (SCs) at the site of nerve injury, where Schwann cell-derived exosomes (SC-Exos) are suspected to exert an induction effect. Our study aimed to induce the differentiation of ADSCs in vitro using SC-Exos and to investigate the mechanisms involved through miRNA sequencing. Methods: Subcutaneous fat was used to extract ADSCs. Exosomes were extracted from Schwann cell lines (RSC96) using ultracentrifugation. After 8 days of induction of ADSCs by SC-Exos, phenotypic characteristics were observed by examining the expression of SC markers (S100, NGFR, MPZ, GFAP) through RT-qPCR, Western blot and immunofluorescence. Additionally, miRNA sequencing was performed on induced ADSCs, followed by bioinformatic analysis and validation of the results. Results: SC-Exos were taken up by human ADSCs. The RNA and protein expression levels of S100, NGFR, MPZ and GFAP were found to be significantly higher in the SC-Exo induction group than in the uninduced group, despite no significant difference to the Dezawa’s method group, which was also consistent with the immunofluorescence results. According to the sequencing results, there were a total of 72 differentially expressed miRNAs. Bioinformatics analysis indicated that 3506 Gene Ontology terms and 98 Kyoto Encyclopedia of Genes and Genomes pathways were significantly enriched. Ten miRNAs, 6 target mRNAs and elevated expression of the PIK3CD/Akt pathway were validated by RT-qPCR or Western blot, which is consistent with the sequencing results. Conclusions: Our data suggest that SC-Exos are able to induce the differentiation of ADSCs into SCs in vitro. It is speculated that the differentially expressed miRNAs play a significant role in the differentiation process.

2022-02-13 | GSE183623 | GEO

Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access

Project description:We describe ‘nanomaterial-biological hybrid complexes’ composed partly of an engineered nanoparticle core and formed partly by active processes within cells. Thus magnetic nanoparticles are internalized by cells, undergo active biomolecular deposition to form a new overlaid cell-derived corona layer before being re-exported. We have characterised the exported nanostructures by RNA-seq and proteomics analysis.

2026-04-16 | E-MTAB-16398 | biostudies-arrayexpress

Schwann cell condition medium MS

Project description:The effects of Schwann cells on the neuro-stroma niche in pancreatic ductal adenocarcinoma (PDAC) remain to be explored. Here, single-cell RNA-sequencing and spatial transcriptome analysis of PDAC tissues reveals that Schwann cells induce malignant subtypes of tumour cells and cancer-associated fibroblasts. Mass Spectrometry (MS) were performed to detected the potential functional factors secreted by Schwann cells.

2023-06-27 | PXD042556 | Pride

Effect of Anesthesia on Force Application During Colonoscopy

Project description:The aim of the study is to determine if more force is used during colonoscopy when patients receive monitored anesthesia with propofol versus conscious sedation. Force measurements will be performed using the colonoscopy force monitor (CFM). This device measures the magnitude and direction of force applied to the insertion tube of a standard colonoscope during colonoscopy.

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Schwann Cell Tn5Prime

Project description:Schwann Cell Tn5Prime

| PRJNA579292 | ENA

Laminin Receptor-Mediated Nanoparticle Uptake by Tumor Cells: Interplay of Epigallocatechin Gallate and Magnetic Force at Nano-Bio Interface.

Project description: Not available

| S-EPMC9330565 | biostudies-literature

Proteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking

Project description:Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron oxide polystyrene nanoparticles and analyzed their protein composition by label free quantitative mass spectrometry. The proteomic snapshot of organelle marker proteins revealed that an atypical macropinocytic-like mechanism mediated the entry of nanoparticles. We show that the entry mechanism is controlled by actin reorganization, atypical macropinocytic signaling and ADP-ribosylation factor 1. Additionally, our proteomics data demonstrated a central role for multivesicular bodies and multilamellar lysosomes in trafficking and final nanoparticle storage. This was confirmed by confocal microscopy and cryo-TEM measurements. By quantitatively analyzing the protein composition of nanoparticle-containing vesicles, our study clearly defines the routes of nanoparticle entry, intracellular trafficking and the proteomic milieu of a nanoparticle-containing vesicle.

2014-09-25 | PXD001175 | Pride

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