Ontology highlight
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PROVIDER: PRJNA1310612 | ENA |
REPOSITORIES: ENA
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Project description:Single-cell RNA-seq (10x) of the goat ovarian granulosa cells samples from high fertility group (>three babys per birth) and low fertility group (< two babys per birth). The goal of this project is to explore the role of ovarian granulosa cells on the fertility of goat at single cell level. We would like to investigate the role of ovarian granulosa cells on the ovulation rate which indicates fertility. Therefore the ovarian granulosa cells from different population (hihg or low fertility groups animals) were analyzed.
2021-12-01 | GSE135688 | GEO
Project description:The aim of this study was to explore DNA methylation status in the ovarian samples of two different population of goat (high fertility and low fertility groups). We profiled DNA methylation sequencing of the goat ovarian samples from high fertility group (>three babys per birth) and low fertility group (< two babys per birth).
2020-08-31 | GSE137099 | GEO
Project description:The product of the Bmal1 locus is an essential component of the circadian clock that plays important roles in various aspects of reproductive biology, and its disruption results in infertility. In an effort to identify the identity of the tissue specific clock that is responsible for this infertility, we used the steroidogenic factor-1 (Sf1) promoter to drive Cre-mediated recombination and genetically delete Bmal1 within cells of the reproductive axis. We show that Bmal1 within the reproductive axis of females is essential for normal fertility through its role in maintaining implantation, but is not required for normal estrous cycling. At the root of this biology appears to be a defect in the regulation of ovarian steroidogenic acute regulator (StAR) and its role in maintaining progesterone synthesis. This conclusion is based upon three observations. First, that deletion of Bmal1 within the reproductive axis leads to lower levels of StAR mRNA, and lower progesterone levels. Second, that progesterone supplementation of these conditional mutants rescues implantation. Third, transplantation of wild type ovaries into Bmal1 reproductive axis mutants results in 100% fertility. Our study suggests that ovarian Bmal1 is an essential peripheral clock governing implantation and fertility in female mice. Ten week old female Bmal1fxfx mice positive or negative for Cre-recombinase driven by the sf-1 promoter, housed in 12 hour light:12 dark, ad lib feeding and drinking conditions were sacrificed at ZT12 on 3.5dpc (3.5 days post copulation). For each array analysis, a pool of 3 RNA samples from 3 individual Bmal1fx/fxCresf-1 ovaries labeled with cy3 were co-hybridized with a pool of 3 RNA samples from Bmal1fx/fx ovaries labeled with cy5, according to Agilent protocols.
2013-02-01 | E-GEOD-38365 | biostudies-arrayexpress
Project description:Ovarian tissue cryopreservation is an important technique for preserving fertility potential, albeit the associated tissue damage may severely impact post-thaw tissue viability. We collected human ovarian tissues from multiple samples and performed Stereo-Seq high-resolution spatial transcriptomics to comprehensively profile and compare the molecular impacts of two cryopreservation methods - slow freezing and vitrification. We identified 8 major spatial clusters and revealed their functional heterogeneity by subclustering. We then detailed cryopreservation response at both the global and subcluster levels to illustrate overall and niche specific effects. Compared to fresh samples, we observed a decrease in major metabolic pathways in frozen samples with both techniques, whereas vitrified samples have severer decrease than slow-frozen samples. The affected metabolic pathways included those related to proteins, such as ribosomal processes and proteasomal degradation; lipids, specifically sterol and cholesterol metabolism; and overall energy production, which encompassed cellular respiration and mitochondrial processes. On the other hand, slow freezing elicited a strong but balanced inflammatory and tissue remodeling state compared to vitrification. We also reported upregulated cell-cell signaling related to angiogenesis, cellular adhesion and extracellular matrix remodeling in slow-frozen tissue. These pathways were responsible for enhancing tissue repair by coordinating with certain stromal and endothelial subclusters. In summary, our study offered insights on ovarian cell response to cryopreservation, which may guide optimization of ovarian tissue cryopreservation protocols for clinical applications.
2024-05-18 | GSE267323 | GEO
Project description:To investigate the mRNA m6A modification profiling in human metastatic ovarian carcinoma and in situ ovarian carcinoma tissue, we performed m6A MeRIP-seq(GenSeq®️ m6A MeRIP Kit) with the total RNA extacted from these tissue samples.
2022-07-15 | GSE208023 | GEO
Project description:The flamenco (flam) locus is the most prominent piRNA cluster locus expressed in Drosophila ovarian follicle cells that is required for female fertility and silencing of gypsy/mdg4 transposons. Although P-element insertions in the flam promoter region disrupts flam piRNAs and cause female infertility, the flam enhancer architecture is still poorly understood. To illuminate flam’s regulation, we uncovered novel shadow enhancer (SE) sequences within the first exons of flam via promoter-bashing assays in OSS cells. We confirmed the SE relevance in vivo with new Drosophila flam deletion mutants of these regions that compromised flam piRNA expression and lowered female fertility from unleashed transposon expression. In a proteomic analysis of proteins associated with these SE sequences, the MAF transcription factor Traffic jam (Tj) emerged as the most prominent binding candidate. With Tj knockdowns in OSS cells, we see a coordinated loss of flam transcripts and piRNAs as well as a decreased expression of many Piwi pathway genes including piwi, which then also coincided with increased transposon RNA levels. We conducted ChIP-seq of Tj’s interaction at Piwi pathway gene promoters and hundreds of other genes important for follicle cell development. Lastly, we utilized transient Tj knockdown in the fly ovary to support our conclusions of how Tj orchestrates the complex Piwi pathway network in Drosophila follicle cells.
2025-04-01 | PXD052946 | Pride
Project description:Malignant tumors, or cancers, are increasingly menacing people's life and health. For your female cancer patient, the ideal therapy should achieve dual purpose: suppress tumor progression meanwhile protect ovarian function. We previously showed that activated (phosphorylated) BIN2 in mouse ovaries regulates primordial follicle activation and oocyte quality through p-RPS6, and in this study, we found that BIN2 knockout or inhibition of BIN2 phosphorylation by BPP could suppress the genesis and progression of ovarian cancer. However, in human female ovarian cancer tissues, we didn’t see the increment of p-RPS6 although we observed a significant raise of p-BIN2. From this discrepancy between normal ovaries and ovarian cancer tissue, we guessed that p-BIN2 has other targets that are more important in ovarian cancer progression. Through mass spec identification, we found that only the constitutively active form of BIN2 (T423D & S424D) baits HDAC1, indicating that HDAC1 is a more important target of BIN2 in ovarian cancer. Next, we did saw Bin2 knockout or inhibition significantly decreased p-HDAC1 (S421) meanwhile increased H3K27ac. Moreover, chip seq showed that BIN2 inhibition significantly increased the binding of H3K27ac to multiple oncogenes. Besides, BIN2 knockout or inhibition could meanwhile protect ovarian function in mice with chemical carcinogen-induced in-situ ovarian cancers or with ovarian cancer cell transplantation. This study suggested that BIN2 inhibition could both suppress ovarian tumorigenesis and protect ovarian fertility, but through distinct mechanisms.
2024-07-01 | GSE244896 | GEO
Project description:The goal of this study was designe to search for the granulosa cells contribution on fertility. Single-cell RNA-seq of the goat ovarian granulosa cells samples from high fertility group (>three babys per birth) and low fertility group (< two babys per birth). And each group was divided into three subgroup based in the size of follicles: large, medium, and small follicles.
2020-08-01 | GSE135897 | GEO
Project description:This study reports role of Jmjd3/kdm6b in female reproduction and provides a molecular insight of how Jmjd3/kdm6b regulates ovarian function and fertility in mice
2022-01-29 | GSE195498 | GEO