Project description:Ovaries transcriptomic profiling between of egg of three high number of laying eggs (HEN) and three low number of laying eggs (LEN) in Longyan Shan-ma duck at 71 weeks were sequenced using Illumina Hiseq 2500 technology.
Project description:The objective of this study was to identify the molecular mechanisms and biological pathways associated with the anticancer effects of flaxseed (richest plant source of Omega-3 fatty acid) in laying hen model of ovarian cancer. Study shows a significant reduction in the severity of the disease and increased survival of the laying hens fed with flaxseed.
2015-05-18 | GSE40376 | GEO
Project description:fecal metagenome of laying hen
Project description:Dongxiang blue-shelled chicken, an indigenous chicken breed in China, has segregated significantly for the dermal hyperpigmentation phenotype. Two lines of the chicken have been divergently selected with respect to comb color for over 20 generations. The recent selection has also resulted in a significant difference in egg production. The red comb line (RCL) chicken produces significantly higher number of eggs than that by the dark comb line (DCL) chicken. The objective of this study was to explore potential mechanisms involved in the relationship between comb color and egg production among chickens. We performed genome-wide association study to identify candidate genes associated with chicken comb color using SNP array data, and we conducted selective sweep analysis to identify putative regions of selection harboring pleiotropic genes affecting both comb color and egg production.
Project description:A total of 565 miRNAs annotated in miRBase 20.0 were identified to be expressed in the liver of hen by high-throughput sequencing three biological reduplication libraries in juvenile and egg-laying hens, respectively. Compared with juvenile hen, 80 miRNAs (67 down-regulated and 13 up-regulated) were verified to be significant differential expression (SDE) in egg-laying physiological stage. Among these, miR-22-3p has the highest abundant expression, and miR-146b-5p has the highest fold-change. Additionally, 19 of the 71 novel miRNAs was significantly expressed. Furthermore, 648 putative target genes of the SDE miRNAs were obtained, and among these, FADS1, FADS2, ELOVL6, ACSL5, etc which are lipid metabolism related critical regulators are targeted by some SDE miRNAs. Gene Ontology (GO) analyses to the putative target genes of all the SDE miRNAs showed significantly enriched in Steroid biosynthesis, Glycerophospholipid metabolism, Biosynthesis of unsaturated fatty acids, and PPAR signaling pathway (P ≤ 0.05). Meanwhile, GO terms are also significantly enriched in lipid related biological processes.
Project description:The laying hen is the only spontaneous model of ovarian tumor. But no studies have systematically compared the molecular mechanisms of ovarian cancer in hens and women. We performed RNA sequencing of nine chicken ovarian tumor samples and four normal ovarian samples.
Project description:Our study investigated the differences of uterine transcriptome in laying hen holding a high or low breaking strength shell. The eggshell calcification periods are divided into three periods, namely initiation, growth and termination periods respectively. The large differences in the transcriptome proved that the initiation period of calcification determine eggshell strength.
Project description:Genome-wide DNA methylation of jejunum mucosa was studied using Reduced Representation Bisulfite Sequencing (RRBS) in two laying hen strains, Lohmann Brown-Classic (LB) and Lohmann LSL-Classic (LSL) collected at 10, 16, 24, 30 and 60 weeks of age.
