Project description:tracheal adenoid cystic carcinoma is one kind of non-small-cell lung cancer with limit treatment options. We used single cell RNA sequencing (scRNA-seq) to analyze the molecular characteristic of tracheal adenoid cystic carcinoma, aiming to recognized this disease deeply and provide potential treatment plan in the future.
Project description:Sox4 is upregulated in adenoid cystic carcinoma (ACC). The ACC3 cell line is the most widely available cell culture model system of this tumor type. RNAi mediated knockdown of Sox4 in ACC3 cells induces apoptosis.
Project description:Human papillomavirus (HPV)-related multiphenotypic sinonasal carcinoma (HMSC) is a recently described rare tumor that is morphologically similar to high grade adenoid cystic carcinoma (ACC), but clinically distinct. We utilized single-cell RNA sequencing (scRNA-seq) to characterize transcriptional heterogeneity in a human HMSC tumor and compared it to published ACC and oropharyngeal squamous cell carcinoma (OPSCC) scRNA-seq datasets.
Project description:Chromatin immunoprecipitation was carried out using an anti-MYB antibody in PDX-derived ACCX11 adenoid cystic carcinoma cells. Input samples were extracted prior to the addition of antibody.
Project description:Lung metastasis is a major factor affecting long-term survival in adenoid cystic carcinoma patients. Here, we report the long noncoding RNA (lncRNA) MRPL23 antisense RNA 1 (MRPL23-AS1), which exhibited remarkably upregulated expression and was correlated with lung metastasis and overall survival in salivary adenoid cystic carcinoma (SACC) patients. To further study which biological process MRPL23-AS1 may be involved in, SACC-83 cells treated with the control vector or MRPL23-AS1-overexpressing vector were subjected to an mRNA microarray. GO analysis was used to identify the significant biological functions of differentially expressed mRNAs.
Project description:Lung metastasis is a major factor affecting long-term survival in adenoid cystic carcinoma patients. Here, we report the long noncoding RNA (lncRNA) MRPL23 antisense RNA 1 (MRPL23-AS1), which exhibited remarkably upregulated expression and was correlated with lung metastasis and overall survival in salivary adenoid cystic carcinoma (SACC) patients. To further study which biological process MRPL23-AS1 may be involved in, SACC-LM cells treated with si-control or si-MRPL23-AS1 were subjected to an mRNA microarray. GO analysis was used to identify the significant biological functions of differentially expressed mRNAs.
