Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA138517 | ENA |
REPOSITORIES: ENA
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Project description:Neonatal genistein treatment causes complete infertility in adult mice. Part of their infertility can be attributed to a loss of 50% of the preimplantation embryos during transit through the oviduct between days 2 and 3 of pregnancy. Comparison of the gene expression signatures of oviducts collected on pregnancy day 2 revealed significant alterations in gene expression, particularly in genes involved in development and the inflammatory response. These findings were verified by real time PCR and in some cases at the protein level by immunoblotting or immunohistochemistry. Mice were treated with either corn oil (control) or genistein 50 mg/kg/day in corn oil for 5 days from postnatal day 1 (day of birth) through postnatal day 5. At 6-8 weeks of age, both groups of mice were superovulated and mated. Vaginal plug-positive mice were euthanized on day 2 of pregnancy (48 hr after hCG administration) and oviducts were collected. Both oviducts from one mouse were combined to form one biological replicate. Four biological replicates from each group (control and neonatal genistein-treated) were compared.
2012-02-21 | E-GEOD-27639 | biostudies-arrayexpress
Project description:Neonatal genistein treatment causes complete infertility in adult mice. Part of their infertility can be attributed to a loss of 50% of the preimplantation embryos during transit through the oviduct between days 2 and 3 of pregnancy. Comparison of the gene expression signatures of oviducts collected on pregnancy day 2 revealed significant alterations in gene expression, particularly in genes involved in development and the inflammatory response. These findings were verified by real time PCR and in some cases at the protein level by immunoblotting or immunohistochemistry.
2012-02-21 | GSE27639 | GEO
Project description:Female mice exposed neonatally to the phytoestrogen genistein (GEN) at doses similar to those in infants consuming soy-based infant formulas are infertile due in part to uterine implantation defects. To determine the mechanisms by which neonatal GEN exposure causes implantation defects, female mice were exposed to vehicle (corn oil) or GEN in corn oil (50 mg/kg/day) on postnatal days (PND) 1-5. Uterine tissues were collected during early gestation for microarray analysis; gestation day 1.5 (GD1.5), GD3.5, GD4.5 and GD5.5. We conclude that neonatal GEN exposure disrupts expression of genes important for uterine development, causing posteriorization and diminished gland function during pregnancy that together cause implantation failure.
2020-01-24 | GSE138500 | GEO
Project description:Our results indicated that conditional knockout of Cdc42 in the epithelial cells of the oviduct isthmus led to defective embryo transport, resulting in blastocytes retention in oviduct, and contributing to the pregnancy failure in PgrCreCdc42f/f mice. To further determine the role of CDC42 in oviduct, RNA-seq analysis was applied in the Cdc42f/f and PgrCreCdc42f/f mice oviduct on day 2 of pregnancy.
2022-07-16 | GSE185465 | GEO
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
2004-01-02 | GSE887 | GEO
Project description:Gene expression in postnatal mammary gland development -pregnancy day 14 and lactation day 2
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