Project description:Pancreatic islets were isolated from Hnf1a (Hepatocyte nuclear factor 1 alpha) knockout and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays [Mouse430A_2].
Project description:Pancreatic islets were isolated from Hnf1a+/- (Hepatocyte nuclear factor 1 alpha) and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].
Project description:Chromatin from mouse hepatocytes was immunoprecipitated with antibodies against Hepatocyte Nuclear Factor 4 alpha (Hnf4a) and IgG. Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.1 arrays.
Project description:Single-nucleus RNA-seq reveals hepatocyte heterogeneity and progenitor-like populations in Lamtor2-deficient mouse liver Single-nucleus RNA sequencing (snRNA-seq) was performed on whole livers from control mice and from two models of hepatocyte-specific Lamtor2 deletion (a chronic developmental knockout and an acute adult knockout). Unsupervised transcriptomic analysis identified multiple liver cell types and hepatocyte subpopulations, revealing that early (developmental) loss of Lamtor2 triggers the emergence of hepatocytes with a biliary/progenitor-like gene expression signature, whereas acute adult deletion leads to a distinct hepatocyte subcluster with altered metabolic gene expression. These findings highlight hepatocyte plasticity and metabolic reprogramming in Lamtor2-deficient livers, underscoring the crucial role of the Ragulator/mTORC1 signaling complex in maintaining normal hepatocyte identity and function.
