Project description:This SuperSeries is composed of the following subset Series: GSE40969: Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq] GSE40994: Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray] Refer to individual Series
Project description:To identify the molecular signature of leucine-activated neurons in the MBH, we adopted the PhosphoTRAP assay following parenchymal injection of leucine into the MBH. Neurons activated following MBH leucine injection (and expressing the neuronal activation marker cFos) co-express Ser240/244-phosphorylated ribosomal S6 protein , allowing selective immunoprecipitation-based capture (TRAP) and RNA sequencing of polysomes from leucine-activated neurons. Differential expression analysis between IP and input samples generated a list of enriched transcripts in leucine-activated neurons
Project description:SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for dissecting the molecular mechanisms behind. Here, we report a novel technique, named DEtail-seq (DNA End tailing followed by sequencing), that can directly and quantitatively capture the meiotic DSB 3’ overhang hotspots at single-nucleotide resolution.
Project description:We use comprehensive and unsupervised transcriptome analyses to provide molecular classifications of sensory neurons in the mouse geniculate ganglion. 96 neurons were isolated on a C1 Fluodigm chip, underwent RNA-Seq, and iteratively clustered into sub-classes.
