Project description:2022_13_qs_umami- umami experiment-Analysis of the effects of biostimulants (amino-acids and humic substances) on plants-Analysis of transcriptomic variations in Arabidopsis seedlings grown in vitro with or without humic substances or amino acid solutions.
Project description:Soil humic substances are known to positively influence plant growth and nutrition. In particular, low-molecular fractions have been shown to increase NO3- uptake and PM H+-ATPase activity and alter expression of related genes. Changes in maize root transcriptome due to treatment with nitrate (NO3-), Water-Extractable Humic Substances (WEHS) and NO3-+WEHS were analyzed.
Project description:Humic substances are a mixture of natural organic macromolecular compounds, mainly fulvic acid (FA) (soluble in alkali, water, and acid) and humic acid (soluble in alkali but insoluble in water and acid). However, to the best of our knowledge, its antitumor effect remains indeterminate. Herein, we aimed to explore the effects and mechanisms of FA in ovarian cancer cells.
Project description:In previous work in our group, shotgun genome sequencing of Arthrobacter sp. revealed potential new P450 monooxygenases and many other oxidoreductases with putative hydroxylation activity. A targeted approach to identify enzymes involved in the degradation of certain molecules is proteomic analysis. In the case of growth on certain substances, enzymes like P450s, which are responsible for the observed organism’s capabilities, might be overexpressed or initially induced.
Project description:The bacterium Novosphingobium sp. THN1 (THN1) is capable of degrading microcystin-LR (MCLR). To get an insight into genes expression during MCLR degradation and the regulation of different carbon concentrations on MCLR degradation, we performed RNA-seq of THN1 during MCLR degradation under different carbon concentrations.
Project description:Humic substances have been widely used as plant growth promoters to improve the yield of agricultural crops. Root soluble protein profiles of 11 days after planting, cultivated with and without humic acids (50 mg C/L), were analysed using the label-free quantitative proteomic approach. The effects on root architecture, such as induction of lateral root and biomass increase were accompanied by changes in the proteins.
Project description:The advances of next-generation sequencing technologies enabled the continuous growth in the number of gene sequences deposited into the CAZy database, meanwhile, the number of biochemically characterized CAZymes is growing at a much slower rate. The biochemical characterization of discovered enzymes is important because of the industrially relevant properties such as high thermal stability or cold-adaptivity that these enzymes may possess. The aim of our project is to screen for up-to-date biochemically uncharacterized CAZymes. We screen for microorganisms that are able to produce extracellular CAZymes. We use LC-MSMS proteomics to identify extracellular enzymes produced by the selected microorganisms.
