Project description:Neuronal Elavl-like (nElavl) RNA binding proteins have three paralogs (Elavl2, Elavl3 and Elavl4) in the mouse genome. This family of RNABPs have been implicated in a variety of post-transcriptional RNA processing mechanisms, including regulation of mRNA stability, alternative splicing, and translational regulation. In this study, using mouse exon arrays, we identify significant differences (p<0.01) in 119 transcripts between wild-type and Elavl3/Elavl4 double knockout forebrain tissue at postnatal day 0. A total of 10 samples were analyzed. These samples consisted of 5 littermate pairs of wild-type and Elavl3/Elavl4 double knockout mice.
Project description:Neuronal Elavl-like (nElavl) RNA binding proteins have three paralogs (Elavl2, Elavl3 and Elavl4) in the mouse genome. This family of RNABPs have been implicated in a variety of post-transcriptional RNA processing mechanisms, including regulation of mRNA stability, alternative splicing, and translational regulation. In this study, using mouse exon arrays, we identify significant differences (p<0.01) in 119 transcripts between wild-type and Elavl3/Elavl4 double knockout forebrain tissue at postnatal day 0.
Project description:We used microarrays to detail the global pattern of gene expression in the cortical regional of MRTF-A/-B double knockout mice at Postnatal day 0 (P0). RNA samples were collected from the cortical regions of control and MRTF-A/-B double knockout mice at P0. Three control MRTFA-/-;MRTF-BFlox/Flox and three MRTF-A-/-;MRTF-BF/F;GFAP-Cre RNA samples were collected and pooled for hybridization on Affymetrix microarrays.
Project description:To elucidate the intricate molecular alterations resulting from Cyfip2 knockout, we conducted single cell transcriptomic analysis on the forebrain tissues of E18.5 Cyfip2 knockout mice and their wild-type littermates. The E18.5 stage was selected due to the nonviability of Cyfip2 knockout mice during the perinatal period. Cyfip2 knockout and wild-type mice were obtained by breeding Cyfip2 heterozygous males and females. For each genotype, we combined the forebrains from two mice.
Project description:We used microarrays to detail the global pattern of gene expression in the cortical regional of MRTF-A/-B double knockout mice at Postnatal day 0 (P0).
Project description:Here we aimed to identify protein interacting partners of the (AU)8 motif mediating mRNA localization. For that, RNA carrying this motifs and its mutated version (ATATATATATATATAT ➝ GTACATACATGTACAT) is included in in vitro produced RNA containing 5 boxB sites, the RNA is incubated with mouse brain lysate and fished out using GRNA chromatography. Bound proteins were analysed by mass spectrometry and ELAVL2, ELAVL3 and ELAVl4 identified among the most enriched interactions of AU)8 motif.
Project description:We generated ELAVL4 knockout (KO), overexpression (OE), and rescue human induced pluripotent stem cell-derived neurons to study the effect that ELAVL4 has on AD-related cellular phenotypes. To gain insight into the molecular cascades involved in neuronal ELAVL4 signaling, we then performed gene expression profiling analysis using data obtained from RNA-seq using triplicate samples for each condition
Project description:We performed unbiased transcriptional profiling in an adult-onset Pkd2 mouse model at an early stage before cysts formed using as controls wild type and PC2+cilia double knockout mice which are protected from ADPKD. By a tripartite comparison, we identified differetially expressed genes which have significant different expression in Pkd2-only knockout with same direction of change from both wild type and Pkd2+Cilia double knockout.
Project description:To determine the requirement for Lyl1 in Lmo2-driven T-ALL, microarray data was perepared from sorted CD4-CD8 double negative thymocytes of wild-type, Lmo2 transgenic and Lmo2-transgenic, Lyl1 knockout mice.
