Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA186420 | ENA |
REPOSITORIES: ENA
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Project description:MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression.
2013-01-12 | GSE43441 | GEO
Project description:MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression. Stomachs were excised immediately following sacrifice, quickly flushed with room-temperature PBS, inflated by duodenal injection of OCT compound, frozen in Cytocool II, and cut into serial 7-M-NM-<m-thick cryosections, which were mounted on Superfrost slides, fixed in 70% EtOH, rehydrated in nuclease-free water, and then incubated in Alexa Fluor 488-conjugated Griffonia simplicifolia GS-II (diluted 1:500 in nuclease-free water) for 15 min. Sections were washed in nuclease-free water and dehydrated in graded ethanol followed by xylene. ZCs were identified as corpus cells that were basal to GS-II labeling and which did not show the dark silhouettes and characteristic shape of parietal cells following xylene dehydration. Four wild-type mice and 5 Mist1M-bM-^HM-^R/M-bM-^HM-^R mice were used for dissection (PixCell II LCM apparatus [7.5-M-NM-<m spot diameter] and CapSure HS LCM caps) to generate two caps per mouse. RNA was purified by PicoPure kit, and RNA integrity was confirmed by an Agilent 2100 Bioanalyzer. All RNA from each cap was treated with DNase I and then reverse transcribed using the SuperScript III (Invitrogen) standard protocol (most cDNA syntheses started with 10 ng of total RNA). Biotinylated cRNA probes were hybridized to the GeneChips. Gene chip arrays used in these experiments were Affymetrix Mouse Gene 1.0ST arrays. Chip quality control and GeneChip to GeneChip comparisons to generate expression profiles were performed using dChip.
2013-01-12 | E-GEOD-43441 | biostudies-arrayexpress
Project description:Continuous regeneration of digestive enzyme (zymogen) secreting chief cells is a normal aspect of stomach function that is disrupted in pre-cancerous lesions. Regulation of zymogenic cell (ZC) differentiation is poorly understood. Here we profile Parietal, Pit, and Zymogenic cells for comparison and study. Keywords: Bhlhb8, Mist1, mucous neck cell, laser-capture microdissection, zymogenic, gastric cell differentiation
2006-10-20 | GSE5018 | GEO
Project description:In this experiment, mucous neck cells from the gastric epithelium of normal, adult C57/B6 mice were laser-capture microdissected to determine gene expression in neck cells relative to pit cells, parietal cells, and zymogenic cells, whose expression profiles were previously deposited in GEO. In this screening experiment, only a single replicate Mouse Genome 430 2.0 GeneChip was generated from laser-capture microdissected cells in the neck region of the gastric unit (identified by positive staining with AlexaFluor-488-GS-II lectin positivity and/or position in the middle of the gastric unit with low labeling with E-cadherin and seconndary Alexafluor-488). RNA was purified by PicoPure kit, RNA integrity verified on Agilent Bioanalyzer, and total RNA pooled from multiple dissections and multiple mice to make ~100 ng RNA, which was then amplirifed, labeled, and fragmented (by Arctureus RiboAmp HS kit followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences). Biotinylated cRNA probes were hybridized to the GeneChips. Given the inevitable contamination of the RNA with RNA from parietal cells, this GeneChip is used only in comparisons with laser-captured parietal cells described in GSE5018 as a baseline reference.
2013-05-31 | E-GEOD-42946 | biostudies-arrayexpress
Project description:Mist1+ cells and parietal cells in mouse stomach were separatedly sorted, and RNAs were isolated. Mist1 (also known as Bhlha15) is expressed in gastric chief cells and gastric stem cells in mice. However, more specific genes for each population needs to be identified to better understand the precise biology in these cell populations. In order to address cell specific gene signature, we separately sorted Mist1+ gastric chief cells and Mist1+ gastric stem cells by FACS, and performed microarray analysis. Mist1+ gastric chief cells were sorted by using Mist1-CreERT; R26-TdTomato mouse stomach, immediately after tamoxifen administration. Mist1+ gastric stem cells were sorted by chief cell-ablated Mist1-CreERT; R26-TdTomato mouse stomach, combining with Lgr5-DTR mice. Lgr5-expressing chief cells were ablated by giving DT into these mice. As a control, acid-secreting gastric parietal cell samples were used. Mice were treated with or without Lgr5-DT ablation before sorting.
2019-06-25 | GSE133205 | GEO
Project description:Here we analyse transcriptome profiles from laser captured lower motor neurons between wild type, heterozygous and homozygous TDP-43 Q331K knockin mice
2018-03-08 | GSE99352 | GEO
Project description:Proteomics of gastric mucosal tissues of H.pylori-infected DDIT4 knockout mice and wild-type mice
2023-12-25 | PXD048124 |
Project description:Purpose: To explore the molecular mechanisms of Mist1+ cells that are specifically resistance to oxidative stress, and the mechanism that Gpx4 knockout (oxidative stress) in regulating the stemness of Mist1+ cells, RNA sequencing was performed to analyze the geome-wide change of Mist1+ cells compared with Mist1- cells, and further the difference between Mist1+ cells and Gpx4 KO-Mist1+ cells. Methods: Total mRNA was extracted from organoids which were isolated from corpus of Mist1Cre/ERT2;RFPLSL and Gpx4F/F;Mist1Cre/ERT2;RFPLSL mice (8 weeks). Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform. Results: Our results revealed differentially expressed genes in Mist1+ cells when compared with Mist1- cells. Further KEGG analysis enriched multiple pathways/biological processes including ROS, endocytosis, oxidative phosphorylation, transcriptional misregulation in cancer, phagosome, cellular senescence, cell cycle and p53 signaling pathway. Further GSEA analysis showed that Mist1 signature genes were highly enriched in the Mist1+ group when compared to the Mist1- group, and Gpx4 knockout further enriched Mist1 signature genes, suggesting a role for Mist1 as a transcriptional factor in determining cellular resistance towards oxidative stress. At the same time, we found that Gpx4 knockout in Mist1+ cells rewired multiple signaling pathways that drive cell growth, especially Hippo signaling pathway that is closely related with gastric cancer initiation and progression Conclusion: Our study present the detailed transcripts analysis of Mist1-, Mist1+ and Gpx4 KO-Mist1+ cells. Based on RNA-seq transcriptome characterization, we conclude a mechanism for Mist1+ cells that are resistance to oxidative stress and act as tumor initiation cells under oxidative stress.
2025-06-24 | GSE277436 | GEO
Project description:In this experiment, mucous neck cells from the gastric epithelium of normal, adult C57/B6 mice were laser-capture microdissected to determine gene expression in neck cells relative to pit cells, parietal cells, and zymogenic cells, whose expression profiles were previously deposited in GEO.
2013-05-31 | GSE42946 | GEO
Project description:Purpose: To explore the molecular mechanisms of Mist1+ cells that are specifically resistance to oxidative stress, and the mechanism that oxidative stress in regulating the stemness of Mist1+ cells, RNA sequencing was performed to analyze the geome-wide change of Mist1+ cells under oxidative stress (high fat diet or Gpx4 knockout) compared with control mice, and further the difference between these two Mist1+ cells. Methods: Total mRNA was extracted from Mist1+ cells which were isolated from corpus of Mist1Cre/ERT2;RFPLSL mice (8 weeks) fed with normal diet and high fat diet. Besides, we formed Mist1+ organoids from Mist1Cre/ERT2;RFPLSL mice and Gpx4F/F;Mist1Cre/ERT2;RFPLSL mice respectively, organoids were passaged, and total mRNA was extracted from organoids in passage 5, 10 and 15. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform. Results: Our results revealed differentially expressed genes in Mist1+ cells from high fat diet-fed mice compared with normal diet-fed mice. Further KEGG analysis enriched multiple pathways/biological processes including ROS, endocytosis, oxidative phosphorylation, Ras signaling, and ferroptosis. Further GSEA analysis showed that genes associated with stem cell maintenance and differentiation were significantly enriched in the high fat diet group, suggesting that Mist1+ cells enhance their stemness and transcriptional activity in response to oxidative stress induced by a high-fat diet. As for Gpx4 knockout-induced oxidative stress, we found that Gpx4 knockout in Mist1+ cells rewired multiple signaling pathways that drive cell growth, especially Hippo signaling pathway that is closely related with gastric cancer initiation and progression. Conclusion: Our study present the detailed transcripts analysis of Mist1+ cells under oxidative stress (high fat diet or Gpx4 knockout). Based on RNA-seq transcriptome characterization, we conclude a mechanism for Mist1+ cells that are resistance to oxidative stress and act as tumor initiation cells under oxidative stress.
2025-06-24 | GSE292919 | GEO