Project description:GLABRA3 (GL3) and GLABRA1 (GL1) function as selector genes that control the differentiation of a group of protodermal cells into trichomes in Arabidopsis thaliana. We performed genome-wide location (ChIP-chip) analyses by using transgenic Arabidopsis plants carrying GL3-YFP or GL1-YFP-MYC1 mini genes. These analyses identified statistically significant enriched DNA associated with GL1 and GL3. A total ~540 and ~700 genes were identified as located proximal and downstream to the GL1 and GL3 binding regions, respectively. Keywords: ChIP-chip
Project description:Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of salt stress RSA3 and used the Affymetrix whole-genome arrays to study the effect of rsa3-1 mutation on global gene expression under salt stress. A set of genes differentially expressed in rsa3-1 under salt stress are identified. Six-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing RD29A::LUC transgene) and rsa3-1 mutant seedlings subjected to salt stress at 120 mM NaCl for 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are two biological replicates per genotype.
Project description:Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified a novel calcium-binding protein and used the Affymetrix whole-genome arrays to define downstream targets of this important protein. We used the microarrays to reveal the effect of rsa1-1 mutation on global gene expression in response to 120 mM Nacl for 0 or 24 h. A set of genes differentially expressed in rsa1-1 with or without salt stress are identified. Six-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing RD29A::LUC transgene) and rsa1-1 mutant seedlings subjected to salt stress with 120 mM NaCl for 0, or 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are three biological replicate per genotype.
Project description:To evaluate the differences in the acclimation of gcd1 gcd3 and WT plants to osmotic stress, we conducted an LC-MS/MS-based label-free quantitative proteomics analysis of WT and gcd1 gcd3 seedlings grown under mock conditions or osmotic stress imposed by 300 mM mannitol treatment. Comparative proteomic analysis of plants treated with osmotic stress reveals the basis of the underlying acclimation mechanism.
Project description:affy_meja_arabidopsis - We want to determine if the presence of C2 modifies the cell transcriptome and its response to methyl jasmonate. Comparison of control lines and transgenic lines expressing C2 in basal conditions and after treatment with 50uM MeJA for 10 hours. C2 lines are expressing geminivirus C2 under a 35S promoter. PR1-LUC have a luciferase transgene under the control of the promoter of PR1; since the plants are not induced, these are the control plants (resistant to kanamycin, but not expressing anything else) Keywords: gene knock in (transgenic),treated vs untreated comparison 12 arrays - ATH1
Project description:Cold stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of cold-responsive genes and used the Affymetrix whole-genome arrays to define downstream targets of this important protein. We used the microarrays to reveal the effect of rcf1-1 mutation on global gene expression with or without cold stress at 4M-BM-0C. A set of genes differentially expressed in rcf1-1 with or without cold stress are identified. Fourteen-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing CBF2::LUC transgene) and rcf1-1 mutant seedlings subjected to cold stress at 4M-BM-0C for 0, 12, and 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are two biological replicate per genotype.
Project description:Investigation of the ecotype difference between Columbia-0 (Col) and Wassilewskija-0 (WS) on whole gene expression level, of PME17 mutation effect (compared to background WS) and of the aphid infestation effect on Col, WS and pme17 (infested plants compared to non-infested plants).
