Project description:Escherichia coli (E. coli) amine oxidase (ECAO) encoded by tynA gene has been one of the model enzymes to study the mechanism of oxidative deamination of amines to the corresponding aldehydes by amine oxidases. The biological roles of ECAO have been less addressed. Therefore we have constructed a gene deletion Escherichia coli K-12 strain, E. coli tynA-, and used the microarray technique to address its function by comparing the total RNA gene expression to the one of the wt. Our results suggest that tynA is a reserve gene for stringent environmental conditions and its gene product ECAO a growth advantage compared to other bacteria due to H2O2 production.
Project description:Protein extract of Escherichia coli K-12 MG1655 was separated by RPLC on a Waters NanoAquity system with a custom packed C5 column and analyzed by an LTQ Orbitrap Velos mass spectrometry. Parent spectra were collected at a 60000 resolution and the top 4 ions were selected for LC-MS/MS analysis in which the resolution was 60000 and the alternating fragmentation mode was used. In total, 2027 collision-induced dissociation (CID) and 2027 electron-transfer dissociation (ETD) top-down MS/MS spectra were collected.
Project description:Successful pathogens must be able to protect themselves against reactive nitrogen species generated either as part of host defence mechanisms, or as products of their own metabolism. The regulatory protein, NsrR (a member of the Rrf2 family of transcription factors), plays key roles in this stress response. Microarray analysis was carried out to reveal the regulon of NsrR. Keywords: Response to repressor titration and different growth conditions
Project description:Expression profiles of wild-type and SgrR mutant E. coli strains under aMG and 2-DG-induced stress. Expression profiles of E. coli overexpressing SgrS sRNA.
