Project description:In vivo profiling of hypoxic gene expression in gliomas using the hypoxia marker EF5 and laser-capture microdissection We have employed the hypoxia marker EF5 coupled with laser capture microdissection to isolate RNA from viable hypoxic and normoxic regions of 9L experimental gliomas.
Project description:E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. Targets from multiple biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430_v2 arrays. Keywords: embryonic metanephric kidney, kidney development, Hoxa11, Hoxd11, compound null targeted mice
2006-05-01 | GSE3822 | GEO
Project description:Laser capture microdissection data
Project description:Laser capture microdissection was used to obtain individual LGN layers for DNA microarray of Rhesus array in macaque monkeys that Monocular Visual Deprivation was generated by either opaque dark contact lens or tarsoraphoplasty at birth. Keywords: Lateral Geniculate Nucleus, monocular deprivation, Rhesus array, Laser Capture Microdissection, Gene expression
Project description:In this study, metabolism dysfunction associated steatohepatitis (MASH) hepatocytes and normal hepatocytes were collected from three MASH mouse models and normal diet control fixed-frozen liver sections with laser capture microdissection (LCM), respectively. Protein was processed from these LCM samples using the HDMSP method. LC-MS/MS analysis was performed with Orbitrap Astral mass spectrometer coupled with Vanquish Neo UHPLC system.
Project description:Through laser capture microdissection and microarray analysis combined with slightly modified RNA extraction and amplification. we could analyze the subtle differential expression between colon normal cell and ulcerative colitis. Keywords: Ulcerative Colitis, amplification, microdissection
