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Cryptococcus neoformans Bt35

ABSTRACT: Cryptococcus

PROVIDER: PRJNA227913 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1063293_1.fastq.gz	Fastqsanger.gz
	SRR1063293_2.fastq.gz	Fastqsanger.gz
	SRR1063294_1.fastq.gz	Fastqsanger.gz
	SRR1063294_2.fastq.gz	Fastqsanger.gz
	SRR1063295_1.fastq.gz	Fastqsanger.gz

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Ribosome profiling of Cryptococcus neoformans H99

Project description:We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.

2019-06-22 | GSE133125 | GEO

CoCl2 Response in Cryptococcus neoformans

Project description:We investigated the effects of the hypoxia-mimetic CoCl2 on the gene expression of pathogenic fungus Cryptococcus neoformans. Keywords: compound treatment design

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Project description:The goal of this study was to position all transcripts extremities in two species of Cryptococcus using TSS-Seq and QuantSeq 3' mRNA-Seq when cells are grown under different conditions. We analysed also the level of expression of each genes in the same condition using the same cell sample. All these data have spiked in using a fixed quantity of S. cereviae cells added just before DNA and RNA extraction.

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Whole genome sequencing of fluconazole adaptors in Cryptococcus neoformans

Project description:Cryptococcus neoformans lab strain H99 was exposed to fluconazole. Adaptors which obtained tolerance to fluconazole were sequenced

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Inositol regulation with Cryptococcus neoformans JEC21 70-mer whole genome array

Project description:Cryptococcus neoformans is the most common cause of fungal meningitis, with high mortality and morbidity. The reason for the frequent occurrence of Cryptococcus infection in the central nervous system (CNS) is poorly understood. In this study, we find that inositol plays an important role in the transversal of Cryptococcus across the blood-brain barrier (BBB) both in an in vitro human BBB model and in vivo animal models. The inositol stimulation of BBB crossing is dependent upon fungal inositol transporters. The upregulation of genes involved in the inositol catabolism pathway is evident in a microarray analysis. The expression of CPS1, a gene encoding the hyaluronic acid synthase in Cryptococcus, is also upregulated by the inositol treatment. The production of hyaluronic acid increased in cells treated with inositol, which leads to the enhanced binding ability of Cryptococcus cells to the human brain microvascular endothelial cells (HBMECs) constituting the BBB. Overall, our studies provide a mechanism for inositol-dependent Cryptococcus transversal of the BBB, supporting our hypothesis that host inositol utilization by the fungus contributes to Cryptococcus CNS infection.

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Whole genome sequencing of benomyl adaptors in Cryptococcus neoformans

Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 4ug/ml benomyl. Randomly 60 adaptors were sequenced.

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Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 2.5ug/ml chlorothalonil. Randomly 72 adaptors were sequenced.

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Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 16 ug/ml azoxystrobin. Randomly 60 adaptors were sequenced.

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Project description:Light is a universal environmental signal perceived by many organisms, including the fungi in which light regulates both common and unique biological processes depending on the species. We conducted a whole-genome microarray analysis on the basidiomycete fungus Cryptococcus neoformans to identify light-regulated genes.

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Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 1.5ug/ml pyraclostrobin. Randomly 23 adaptors were chosen. These adaptors were sequenced.

2023-04-06 | E-MTAB-12415 | biostudies-arrayexpress

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