Project description:we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera ‘Cabernet sauvignon’ and Vitis aestivalis ‘Norton’ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Keywords: time course
Project description:Genome-wide expression profiling of Vitis vinifera L. cv. Cabernet Sauvignon (CS) and two rootstocks, Kober 5BB and 41B, exposed to seven days of water deficit or to increasing salinity reaching 120 mM NaCl. Shoot tissue was profiled on the Affymetrix GeneChip Vitis vinifera Genome Array to identify shared and genotype-specific transcriptional responses to the two stresses.
Project description:Methods:transcriptomes of the different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were analyzed using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed: TopHat followed by Cufflinks. mRNA profiles of different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500.
Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
Project description:To investigate differentially accumulated proteins caused by transgenic events, comparative proteomic analysis using two-dimensional polyacrylamide gel electrophoresis was employed for Vitis vinifera ‘Thompson seedless’ leaves and transgenic Vitis vinifera ‘Thompson seedless’ leaves. Obtained proteins were digested with trypsin and subjected to MALDI-TOF-MS/MS analysis. According to annotations and a Blast search, proteins analyzed by MALDI-TOF/TOF were classified into 4 functional categories: carbon and energy metabolism, oxidation reaction, protein metabolism and resistance.
Project description:Methods:transcriptomes of the different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were analyzed using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed: TopHat followed by Cufflinks.
Project description:RNA-seq was used to assess gene expression variation in transgenic calli lines expressing Vitis vinifera chardonnay VvSWEET10 gene.
