Project description:Standardized skin wounds were established surgically on mice and allowed to heal during a 15-day period. Expression of genes related to heparan sulfate biosynthesis was studied in wound bed and edges during the healing process. Total RNA was isolated from wound edge (regenerating skin) and wound bed at 2, 6 and 15 days post wounding, as well as from intact control skin. Three animals were used for each time point.
Project description:To understand the molecular mechanism of minority cell population in skin would healing process, we performed single cell RNA sequencing (scRNA-seq) of murine skin wound tissues at 3 different time points after injury (3d, 7d, 14d).
