Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.
Project description:We investigated in mouse models how enhanced coagulation activation due to a common disease polymorphism in coagulation factor V (fV Leiden Arg506Gln) modifies the host response to infection and inflammation We used microarrays to characterize the in vivo and in vitro inflammatory responses of mouse spleen dendritic cells, macrophage-like RAW264.7 cells, and bone marrow myeloid cells Samples 1-6 were generated by pooling equal amounts of RNA prepared from FACS-enriched spleen dendritic cells isolated from 6 individual mice before (control sample 1) or 16 hours after challenge with lipopolysaccharide. Samples 7-10 were generated by pooling equal amounts of RNA prepared from triplicate cultures of RAW264.7 cells. Samples 10-14 (2 replicas per condition) were prepared from RNA isolated from FACS-enriched bone marrow myeloid cells (pooled from 4 adoptively transferred animals each) 7 days after infection with S.aureus.
Project description:We investigated in mouse models how enhanced coagulation activation due to a common disease polymorphism in coagulation factor V (fV Leiden Arg506Gln) modifies the host response to infection and inflammation We used microarrays to characterize the in vivo and in vitro inflammatory responses of mouse spleen dendritic cells, macrophage-like RAW264.7 cells, and bone marrow myeloid cells
Project description:Classical dendritic cells may be found in mouse bone marrow and spleen. Due to the differences in their local environment, two populations may express different genes and potentially carry different functions We used microarrays to compare the gene expression profiles between myeloid dendritic cells and classical dendritic cells in spleen. Our data supported the hypothesis that bone marrow myeloid dendritic cells are enriched for the expression of certain sets of genes that may play specific functions in the bone marrow microenvironment
