Mus musculus
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ABSTRACT:
PROVIDER: PRJNA258167 | ENA |
REPOSITORIES: ENA
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Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
2004-01-02 | GSE887 | GEO
Project description: Not available
| S-EPMC8784167 | biostudies-literature
Project description:Cementoblasts are cells that line the surface of the tooth root and produce a mineralized tissue known as cementum. Cementum is critical for attachment of the tooth to the adjacent periodontal ligament and has a similar biochemical composition to bone, however it differs in that it lacks vasculature and innervation, and does not undergo remodelling. It has been difficult to define markers that specifically identify cementoblasts. In order to compare the transcriptome of fully developed cementoblasts with neural crest derived (calvarial) osteoblasts in vivo, we used osteocalcin-GFP (OC-GFP) mice. Cells were isolated by digestion of calvaria (osteoblasts) or extracted molars (cementoblasts) and purified by FACS sorting for GFP positive cells before RNA extraction and microarray analysis.
2014-12-31 | GSE60394 | GEO
Project description:Immediate Effects of Extracellular Phosphate on Gene Expression in Primary Murine Osteoblasts
| PRJNA205904 | ENA
Project description:Gene expression profiles of murine fibroblasts and iPS
| PRJNA380813 | ENA
Project description:Gene expression profiles in TNBS-induced murine colitis
| PRJNA97275 | ENA
Project description: Not available
| S-EPMC11335981 | biostudies-literature
Project description:Simulated Microgravity Inhibits Differentiation and Alters Gene Expression Profiles of 2T3 Pre-osteoblasts
| PRJNA90125 | ENA
Project description:The importance of unanchored Ub in innate immunity has been shown only for a limited number of unanchored Ub-interactors. We investigated what additional cellular factors interact with unanchored Ub and whether unanchored Ub plays a broader role in innate immunity. To identify unanchored Ub-interacting factors from murine lungs, we used His-tagged recombinant poly-Ub chains as bait. These chains were mixed with lung tissue lysates and protein complexes were isolated with Ni-NTA beads. Sample elutions were subjected to mass spectrometry (LC-MSMS) analysis.
2022-02-28 | PXD031365 | Pride
Project description: Not available
| S-EPMC5321798 | biostudies-literature