Project description:We provide a comprehensive single cell mRNA-seq analyses of mouse bone marrow progenitors revealing early lineage commitment in the bone marrow.
Project description:Erythropoiesis is the fabrication process of erythrocytes and is located in bone marrow. After commitment, erythroid progenitors differentiate into erythroblasts and generate immature reticulocytes. Reticulocytes are then released in the blood circulation and mature into ertythrocytes. While absolute quantification of the proteome of erythroblasts, circulating reticulocytes and ertythrocytes have already been studied, a few is known about immature reticulocytes located in the bone marrow. Here, we mesured the absolute quantification of immature bone marrow reticulocytes and compare it to mature circulating reticulocytes and erythrocytes.
Project description:Understanding how cellular function is imprinted during development requires the identification of factors controlling lineage specification and commitment, and the intermediate progenitors in which they act. Using population level and single cell approaches, we examine transcriptional and functional heterogeneity within early innate lymphoid cells (ILC) progenitors. We identify a developmental bifurcation toward dendritic cell fate that reveals the uncommitted state of early specified ILC progenitors. We subsequently characterize an ILC-commitment checkpoint controlled by the transcription factor TCF-1. The present study reveals unexpected heterogeneity within early innate progenitor populations, and characterizes lineage infidelity that accompanies early ILC specification prior to commitment.
Project description:Understanding how cellular function is imprinted during development requires the identification of factors controlling lineage specification and commitment, and the intermediate progenitors in which they act. Using population level and single cell approaches, we examine transcriptional and functional heterogeneity within early innate lymphoid cells (ILC) progenitors. We identify a developmental bifurcation toward dendritic cell fate that reveals the uncommitted state of early specified ILC progenitors. We subsequently characterize an ILC-commitment checkpoint controlled by the transcription factor TCF-1. The present study reveals unexpected heterogeneity within early innate progenitor populations, and characterizes lineage infidelity that accompanies early ILC specification prior to commitment.
Project description:This SuperSeries is composed of the following subset Series: GSE34892: IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Affymetrix). GSE34915: IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Illumina). Refer to individual Series
Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.
