Project description:GSH, being a versatile molecule, is actively involved in various bilogical processe of plant system. Our previous studies identifies an active role of GSH in plant defense signaling network. Here, we used microarray under GSH treated condition to obtain a global expression profiling under this altered GSH conditions.
Project description:GSH, being a versatile molecule, is actively involved in various bilogical processe of plant system. Our previous studies identifies an active role of GSH in plant defense signaling network. Here, we used microarray under GSH treated condition to obtain a global expression profiling under this altered GSH conditions. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. A.thaliana, much accalimed model system of plant biology and being fully sequenced, we used this system to explore the specific relation of GSH with metabolic processes, phisiological conditions, etc.
Project description:This project aimed to identify proteins interacting with metacaspase 3 from Arabidopsis thaliana under regular growth conditions and
Project description:Identification of differentially expressed genes in Arabidopsis thaliana mutants in response to combined abiotic stress treatment through Microarray experiment.
Project description:To comprehensively investigate the effects of glutathione on the gene expression, the microarray analysis was performed in the glutathione-fed wild-type Arabidopsis thaliana. Wild-type Arabidopsis (ecotype Columbia-0) were fed with 1 mM oxidized glutathione (GSSG) and 2 mM reduced glutathione (GSH) for comparison at equal nitrogen equivalents. To examine the effects of glutathione other than nitrogen at equal nitrogen equivalents, plants were fed with 3 mM NH4NO3. Plants grown by water were used as a control.
Project description:In order to explore the regulatory network of plant autophagy, this study generated stable transgenic lines expressing ATG5-TurboID, ATG7-TurboID and ATG11-TurboID. Autophagy was induced by combined treatment with the autophagy inducer AZD8055 and biotin. Proximal proteins of autophagy-related proteins under autophagic conditions were enriched using immunoprecipitation coupled with mass spectrometry (IP-MS), followed by network analysis to identify potential interacting proteins and functional modules. This study provides a richer range of candidate targets for further dissecting the autophagy regulatory network in Arabidopsis thaliana.
Project description:Keeping imbibed seeds at low temperatures for a certain period, so called seed vernalization (SV) treatment, promotes seed germination and subsequent flowering in various plants. Vernalization-promoting flowering requires GSH. However, the expression patterns analyzed by GeneChip arrays showed that increased GSH biosynthesis partially mimics SV treatment in Arabidopsis thaliana. SV treatment (keeping imbibed seeds at 4°C for 24 h) induced a specific pattern of gene expression and promoted subsequent flowering in wild-type plants. A similar pattern was observed at 22°C in transgenic plants (35S-GSH1 plants) overexpressing the γ-glutamylcysteine synthetase gene GSH1, coding an enzyme limiting GSH biosynthesis, under the control of the cauliflower mosaic virus 35S promoter. This pattern was strengthened at 4°C but flowering was less responsive to SV treatment. There was a difference in the transcript behaviour of the flowering repressor FLC between wild-type and 35S-GSH1 plants. Unlike other genes responsive to SV treatment, SV-dependent decrease in FLC in wild-type plants was reversed in 35S-GSH1 plants. SV treatment increased GSSG level in wild-type seeds, whereas GSSG level was high in 35S-GSH1 plants, even at a non-vernalizing temperature. Taking into consideration that low temperatures stimulate GSH biosynthesis and bring about oxidative stress, GSSG is considered to trigger low temperature response, but enhanced GSH synthesis was not enough for mimicking SV treatment. To complete it, it essentially required the cellular redox retransition from the oxidized to the reduced state that is observed after the seed vernalization treatment.
