Project description:RNA-seq was carried out on prostate tumors from mice with prostate-conditional PTEN knockout with and without prostate-conditional FOXP1-SHQ1 locus deletion (12 month old PTEN-flox/flox Pb-Cre4 mice and FOXP1-SHQ1-flox/flox PTEN-flox/flox Pb-Cre4 mice )
Project description:This study investigates age-related alterations in the brain proteome of mice using quantitative proteomics. Brain tissues from young (2-month-old) and aged (24-month-old) mice were solubilized with PTS buffer, and the extracted proteins were subjected to tandem mass tag (TMT) labeling and mass spectrometry analysis.
Project description:Gene expression analysis of 2-month-old APP/APLP2 double-conditional Knockout (N-dCKO) mice and littermate APLP2 knockout controls, APP knockout and wildtype controls. Mouse hippocampus were dissected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:This study investigates the role of oligodendroglial Serpina3n in brain aging by examining its depletion using Olig2-Cre Serpina3n flox/flox mice model. To assess the molecular impact of oligodendroglial Serpina3n depletion on brain aging, RNA-seq was performed on forebrain samples from three experimental groups: 1) Young Wild-Type Mice (2-month-old): Representing baseline gene expression in a young, healthy brain; 2) Aged Wild-Type Mice (22-month-old): Serving as a control group to analyze age-related changes in gene expression without genetic modifications. 3) Aged Olig2-Cre Serpina3n flox/flox mice (22-month-old): Assessing the effects of oligodendroglial Serpina3n depletion on the transcriptomic profile of aged brains. The experiment aims to identify gene expression changes associated with normal brain aging by comparing young and aged wild-type mice, determine the specific impact of oligodendroglial Serpina3n depletion on the aged brain by comparing aged wild-type mice to aged knockout mice, and highlight molecular mechanisms influenced by Serpina3n in the context of brain aging. This design allows for a comparative analysis of aging-related transcriptional changes and the specific contribution of Serpina3n depletion in this process.
