Project description:The goal of this experiment was to distinguish those genes regulated following acute HNF4alpha depletion compared to a developmental knockout model where gene compensation may comfound results. Expression profile of livers from 8 week old male Hnf4alpha Flox mice that express either albumin cre or the tamoxifen inducible ErT2-albumin cre for liver-specific deletion. Mice were fed a control diet or tamoxifen diet in the case of the ErT2-albumin cre to induce recombination.
Project description:Gene expression differences between wild-type and liver-specific Hnf4alpha knockout (Albumin Cre+/-; Hnf4 fl/fl) mouse liver were assessed using RNA obtained from 2-month-old control and genetically modified female mice. RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].
Project description:The goal of this experiment was to distinguish those genes regulated following acute HNF4alpha depletion compared to a developmental knockout model where gene compensation may comfound results. Expression profile of livers from 8 week old male Hnf4alpha Flox mice that express either albumin cre or the tamoxifen inducible ErT2-albumin cre for liver-specific deletion. Mice were fed a control diet or tamoxifen diet in the case of the ErT2-albumin cre to induce recombination. Six-condition experiment, H4N vs H4T vs H4EN vs H4ET vs H4CN vs H4CP. Biological replicates: 4 samples each group.
