Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA267858 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The root cap surrounds the root meristem, protecting it from harsh environments and facilitating root movement through soil. In Arabidopsis, new root cap cells produced in the meristem displace older cells toward the root periphery, where they undergo programmed cell death and are released from the root. This rapid cell turnover is a unique feature of the root cap and is modulated in part by the combined action of four NAC transcription factors, as well as cell wall modification enzymes. Here we show that the transcription factor NIN-LIKE PROTEIN 7 (NLP7) regulates root cap maturation in Arabidopsis by modulating expression of each of the NAC TFs as well as several cell wall modifying enzymes. NLP7 is a homolog of NIN, a transcription factor required for nodulation in Lotus japonicas, and is highly expressed in the columella root cap. Mutants have altered columella root cap development and decreased levels of homogalacturonan, a major component of pectin. Reverse genetic analysis of genes differentially expressed in nlp7 roots showed that two cell wall modifying enzymes, CELLULASE5 and XTH5, modulate root cap maturation likely downstream of NLP7. Plant cell wall modification is critical for nodulation, and we propose that this characteristic is also present in NLPs. We used microarrays to identify the differences in gene expression between whole roots of WT and nlp7-1 Arabidopsis plants
2016-07-31 | GSE63474 | GEO
Project description:Chromatin immunoprecipitation was performed in nlp7-1 Arabidopsis thaliana seedlings complemented by a pNLP7::NLP7-GFP construct upon 10 minutes NO3- resupply after a 3-day NO3- starvation.
2013-03-23 | GSE36763 | GEO
Project description:The root cap surrounds the root meristem, protecting it from harsh environments and facilitating root movement through soil. In Arabidopsis, new root cap cells produced in the meristem displace older cells toward the root periphery, where they undergo programmed cell death and are released from the root. This rapid cell turnover is a unique feature of the root cap and is modulated in part by the combined action of four NAC transcription factors, as well as cell wall modification enzymes. Here we show that the transcription factor NIN-LIKE PROTEIN 7 (NLP7) regulates root cap maturation in Arabidopsis by modulating expression of each of the NAC TFs as well as several cell wall modifying enzymes. NLP7 is a homolog of NIN, a transcription factor required for nodulation in Lotus japonicas, and is highly expressed in the columella root cap. Mutants have altered columella root cap development and decreased levels of homogalacturonan, a major component of pectin. Reverse genetic analysis of genes differentially expressed in nlp7 roots showed that two cell wall modifying enzymes, CELLULASE5 and XTH5, modulate root cap maturation likely downstream of NLP7. Plant cell wall modification is critical for nodulation, and we propose that this characteristic is also present in NLPs. We used microarrays to identify the differences in gene expression between whole roots of WT and nlp7-1 Arabidopsis plants Roots were cut at the root/hypocotyl junction and collected into RLT buffer in the RNeasy plant mini kit. Approximately 60 roots were collected per replicate, with two biological replicates. RNA was extracted using the RNeasy Micro Kit. Probes for array analysis were preparedfrom 1μg total RNA with the one-cycle amplification protocol by Affymetrix according to the manufacturer's instructions. Samples were submitted to Expression Analysis Inc. (Durham, NC) for hybridization to Arabidopsis Whole Genome ATH1 Affymetrix GeneChips.
2016-07-31 | E-GEOD-63474 | biostudies-arrayexpress
Project description:Chromatin immunoprecipitation was performed in nlp7-1 Arabidopsis thaliana seedlings complemented by a pNLP7::NLP7-GFP construct upon 10 minutes NO3- resupply after a 3-day NO3- starvation. Genome-wide profiling of NLP7 binding regions by ChIP-chip, 2 biological replicates in dye-swap
2013-03-23 | E-GEOD-36763 | biostudies-arrayexpress
Project description:Expression data from MTF mutant roots
2015-05-11 | E-GEOD-51236 | ExpressionAtlas
Project description:Transcriptional profiling of Arabidopsis wild-type (Col0) control seedlings with corresponding mutant seedlings is performed using Aligent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K).
2017-02-09 | GSE39898 | GEO
Project description:Transcriptional profiling of Arabidopsis wild-type (Col0) control flower buds or seedlings with corresponding mutant flower buds or seedlings is performed using Aligent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K).
2020-02-01 | GSE94731 | GEO
Project description:Analysis of gene expression in the meristematic zone of Arabidopsis roots overexpressing miR396
2015-11-18 | GSE58807 | GEO
Project description:Transcriptional profiling in young flowers (stage 8) of Arabidopsis wild type control plants and sdg2-1 mutant is performed using Aligent’s Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K). A significant number of genes involved in gametophyte development are found differentially regulated in the sdg2-1 mutant.
2010-10-06 | GSE18513 | GEO
Project description:Plants modulate gene expression profile in response to nitrate through NIN-LIKE PROTEIN (NLP) transcription factors in order to promote nitrate uptake and utilization. Although there are 9 NLP proteins in Arabidopsis, NLP7 had attracted most of the attention. Here, we focused on NLP2, because the nlp2 and nlp7 knockout mutants displayed different phenotypes. To gain insight into genes whose expression was affected by the nlp2 mutation, we compared gene expression profiles in the wild-type Columiba and the nlp2 mutant that were treated with 10 mM KNO3 for 1 hour. Columbia and nlp2 that were treated with 10 mM KCl for 1 hour were used as controls. We found that nitrate induction of 6 genes was compromised only in the nlp2 mutant.
2021-06-01 | GSE152411 | GEO