Project description:The abscence of TBR2 gene in human leads to microcephaly. This condition is mimicked by the specific ablation of the murine gene in developing cerebral cortex. Herein we compared gene expression in control and Tbr2 cKO in E14.5 cerebral cortices. This approach represents a useful tool to identify the molecular mechanisms at the basis of the phenotype. 6 samples, 3x Tbr2 +/+;Foxg1::Cre (control) and 3x Tbr2 fl/fl;Foxg1::Cre
Project description:The abscence of TBR2 gene in human leads to microcephaly. This condition is mimicked by the specific ablation of the murine gene in developing cerebral cortex. Herein we compared gene expression in control and Tbr2 cKO in E14.5 cerebral cortices. This approach represents a useful tool to identify the molecular mechanisms at the basis of the phenotype.
Project description:This is RNA sequencing performed with embryonic day 12 (E12) microdissected mouse cortices with the following genotypes: Emx1 Cre/ +; Zbtb7a +/+ (WT), Emx1 Cre/+; Zbtb7a fl/+ (cHet) and Emx1 Cre/+; Zbtb7a fl/fl (cKO). The goal of this study was to understand the impact ot Zbtb7a knockout during cortical development.
Project description:β-catenin signaling is required for hair follicle development, but it is unknown whether it is sufficient to activate expression of hair follicle genes in embryonic skin. To address this we profiled gene expression in skin dissected from E14.5 KRT14-Cre Ctnnb1(Ex3)fl/+ embryos carrying an activating mutation in epithelial beta-catenin, and control littermate embryos. Experiment Overall Design: Total skin RNA from two KRT14-Cre Ctnnb1(Ex3)fl/+ and two control littermate E14.5 embryos was hybridized to Affymetrix GeneChip Mouse Genome MOE430 2.0 oligonucleotide microarrays. Experiment Overall Design: Appended below is Table S3: Full list of differentially expressed genes in KRT14-Cre Ctnnb1(Ex3)fl/+ mutant compared with control littermate intact skin at E14.5, including normalization and filter parameters. Fold change, listed in the second column, gives the ratio of normalized mutant : control transcript levels.
Project description:This is RNA sequencing performed with embryonic day 12 (E12) microdissected mouse cortices with the following genotypes: Emx1 Cre/ +; Zbtb7a +/+ (WT) and Emx1 Cre/+; Zbtb7a fl/fl (cKO). The goal of this study was to understand the impact ot Zbtb7a knockout during cortical development.
Project description:To investigate the gene expression patterns in Foxg1 frameshift mutation, we performed RNAseq in P1 cortices of Q84Pfs heterozygote and littermate wild type control mice.
Project description:Taf8 was deleted in the mouse central nervous system using our Taf8 conditional allele. To determine transcriptional changes due to Ta8 deletion in the developing CNS, unstranded RNA sequencing was performed on cortices from Taf8 deleted embryos (E14.5 Taf8lox/–NesCreT/+p53–/–) and control embryos (E14.5 Taf8+/+NesCreT/+p53–/–).
